Production of poly-γ-glutamic acid by submerged fermentation with Bacillus subtilis natto
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- Received Date:
January 31, 2012
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Abstract
Production of poly-γ-glutamic acid (γ-PGA) by cultivation of Bacillus subtilis natto CICC 20643 in submerged state had been studied.The optimum cultivation conditions through single factor and orthogonal experiments were set as follows:sucrose 25g/L, yeast extract 5g/L, glutamic acid 40g/L, initial pH at 6.5, culture volume 70mL in 250mL conical flask.Under the optimum cultivation conditions, B.subtilis natto was shaking cultivated at 37℃, 120r/min for 24h, then 5%NaCl was added, and subsequently cultivated for another 24h.As a result, the yield ofγ-PGA was got at 18.04g/L.The experimental result showed that B.subtilis natto CICC 20643 was a strain of high-yieldγ-PGA and addition of NaCl in the course of cultivation could increase the yield ofγ-PGA.
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References
| [1] |
Shih I L, Van Y T.The production of poly- (γ-glutamic acid) from microorganisms and its various applications[J].Bioresource Technology, 2001, 79:207-225.
|
| [2] |
李晶博, 李丁, 邓毛程, 等.γ-聚谷氨酸的特性生产及应用[J].化工进展, 2008, 27 (11) :1789-1799.
|
| [3] |
Tin O, Alexander S.Microbial degration of poly-amino acids[J].Biomacro molecules, 2004, 5:1166-1176.
|
| [4] |
陈咏竹, 孙启玲.γ-多聚谷氨酸的性质发酵生产及应用[J].微生物学通报, 2004, 31 (1) :122-126.
|
| [5] |
Wu Q, Xu H, Xu L, et al.Biosynthesis of poly (γ-glutamic acid) in Bacillus subtilis NX-2:Regulation of stereochemical compositionof poly (g-glutamic acid) [J].Process Biochemistry, 2006, 41:1650-1655.
|
| [6] |
李大力, 詹长娟, 郄丽, 等.盐浓度对纳豆芽孢杆菌发酵产γ-聚谷氨酸影响的研究[J].化学与生物工程, 2007, 24 (2) :50-51.
|
| [7] |
Shih I L, Van Y T, Chang Y N.Application of statistical experimental methods to optimize production of poly (γ-glutamic acid) by Bacillus licheniformis CCRC12826[J].Enzyme and Microbial Technology, 2002, 31:213-220.
|
| [8] |
Du G, Yang G, Qu Y, et al.Effects of glycerol on the production of poly (g-glutamic acid) by Bacillus licheniformis[J].Process Biochemistry, 2005, 40:2143-2147.
|
| [9] |
桑莉, 徐虹, 李晖, 等.γ-聚谷氨酸产生菌的筛选及发酵条件[J].过程工程学报, 2004, 4 (5) :462-466.
|
-
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