Determination on lycopene in functional foods by the high performance liquid chromatographic method

The content of lycopene in functional foods was determined by the high performance liquid chromatographic method (HPLC) .The sample pre-treatment method was by use of water-oil two-phase extraction methods.Separation was achieved by the use of Inertsil ODS-SP column (5μm, 4.6×250mm) , acetonitrile:methanol (1:1) as mobile phase, a flow rate of 1.2mL/min and detector at 472nm was used.Lycopene showed good linear relationship between the range of 0.30 and 50.0μg/mL (R2=0.9998) ;and the average recovery was 98.6%with RSD=3.0%.The results showed that the method was accurate.