噬菌体展示技术在食品安全分析中的应用

赵岩岩; 赵圣明; 梁颖; 刘贤金; 张浩; 李红波; 屠康

doi:10.13386/j.issn1002-0306.2017.14.067

噬菌体展示技术在食品安全分析中的应用

1. 河南科技学院食品学院
2. 江苏省农业科学院食品质量安全与检测研究所
3. 南京农业大学江苏省肉类生产与加工质量安全控制协同创新中心

基金项目:

河南省高校科技创新团队支持计划(16IRTSTHN007)；河南省科技攻关项目(162102110065,172102310240)；河南科技学院高层次人才科研项目(2016019)；

详细信息

作者简介:
赵岩岩 (1987-) , 女, 博士, 讲师, 研究方向:微生物与生物技术, E-mail:zhaoyanyan@hist.edu.cn。;

屠康 (1968-) , 男, 博士, 教授, 研究方向:农产品无损检测、农产品贮藏与加工, E-mail:kangtu@njau.edu.cn。;

中图分类号: Q819;TS201.6
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- 收稿日期: 2016-12-19

Application of phage display technology in food safety

Department of Food Science, Henan Institute of Science and Technology
Ministry of Agriculture Institute of Food Quality Safety and Detection, Jiangsu Academy of Agricultural Sciences
Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University

摘要

摘要: 随着噬菌体展示技术的发展,其在食品安全领域的应用也越来越多,该技术可以作为一种高效的抗体制备技术应用于食品中常见的抗生素、生物毒素、有害小分子的检测及食源性致病菌控制,同时可以开发有毒待检物的替代品建立绿色检测技术。此外,噬菌体展示技术在新型食品防腐剂开发等方面也具有广阔的应用前景。本文在介绍噬菌体展示技术的基础上,对其在食品安全领域的应用进行了综述。
- 噬菌体展示技术 /
- 高通量筛选 /
- 食品安全 /
- 检测
Abstract: In this article, the phage display technology was described, and its application in food safety was summarized. With the development of the phage display technology, it is widely used in the food safety. It can be used as an efficient method of generation of antibody to detect antibiotic, bio-toxin and small molecule in food, used to screening mimic for developing green detection technology, and the control of foodborne pathogenic bacterial. In addition, this technology also showed broad application in the field of developing novel food preservatives.
- phage display technology /
- high throughput screening /
- food safety /
- detection

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参考文献(45)

[1]	PANDE J, SZEWCZYK M M, GROVER A K.Phage display:Concept, innovations, applications and future[J].Biotechnology Advances, 2010, 28 (6) :849-858.
[2]	FREI J C, LAI J R.Protein and antibody engineering by phage display[J].Methods in Enzymology, 2016, 580:45-87.
[3]	CRUZ S D L, CUBILLOS-ZAPATA C, LóPEZ-CALLEJA I M, et al.Isolation of recombinant antibody fragments (sc Fv) by phage display technology for detection of almond allergens in food products[J].Food Control, 2015, 54:322-330.
[4]	CLEMENTI N, MANCINI N, SOLFOROSI L, et al.Phage display-based strategies for cloning and optimization of monoclonal antibodies directed against human pathogens[J].International Journal of Molecular Sciences, 2012, 13 (7) :8273-8292.
[5]	CRUZ S D L, CUBILLOS-ZAPATA C, LóPEZ-CALLEJA I M, et al.Isolation of recombinant antibody fragments (sc Fv) by phage display technology for detection of almond allergens in food products[J].Food Control, 2015, 54:322-330.
[6]	RAHBARNIA L, FARAJNIA S, BABAEI H, et al.Evolution of phage display technology:from discovery to application[J].Journal of Drug Targeting, 2017, 25 (3) :216-224.
[7]	GULIG P A, MARTIN J L, MESSER H G, et al.Phage display methods for detection of bacterial pathogens[M].New York:Springer, 2007:755-783.
[8]	CHEN X, DRESKIN S C.Application of phage peptide display technology for the study of food allergen epitopes[J].Molecular Nutrition and Food Research, 2016:1600568.
[9]	MARUYAMA I N, MARUYAMA H I, BRENNER S.Lambda foo:a lambda phage vector for the expression of foreign proteins[J].Proceedings of the National Academy of Sciences, 1994, 91 (17) :8273-8277.
[10]	REN Z J, BLACK L W, LEWIS G K, et al.Phage display of intact domains at high copy number:a system based on SOC, the small outer capsid protein of bacteriophage T4[J].Protein Science, 1996, 5 (9) :1833-1843.
[11]	ROSENBERG A, GRIFFIN K, STUDIER F W, et al.T7select phage display system:a powerful new protein display system based on bacteriophage T7[J].Innovations, 1996, 6:1-6.
[12]	YUAN S L, LI X, CHEN H B, et al.Research progress in the phage display technology mapping epitope for major food allergens[J].Science and Technology of Food Industry, 2015, 36 (3) :379-384.
[13]	WINTER G, GRIFFITHS A D, HAWKINS R E, et al.Making antibodies by phage display technology[J].Annual Review of Immunology, 1994, 12 (1) :433-455.
[14]	TAN Y, TIAN T, LIU W, et al.Advance in phage display technology for bioanalysis[J].Biotechnology Journal, 2016, 11 (6) :732-745.
[15]	FUKUDA M N.Screening of peptide-displaying phage libraries to identify short peptides mimicking carbohydrates[J].Methods Enzymol, 2006, 416:51-60.
[16]	DüBEl S, REICHERT J M.Handbook of therapeutic antibodies[M].Second edition, Wiley:Wiley-VCH Verlag Gmb H and Co.KGa A:2014:43-76.
[17]	BURMESTER J, PUGLIESE L K A, HONEGGER A, et al.Selection, characterization and X-ray structure of anti-ampicillin single-chain Fv fragments from phage-displayed murine antibody libraries[J].Journal of Molecular Biology, 2001, 309 (3) :671-685.
[18]	ZHANG X, Zhang C, LIU Y, et al.Construction of sc Fv phage display library with hapten-specific repertories and characterization of anti-ivermectin fragment isolated from the library[J].European Food Research and Technology, 2010, 231 (3) :423-430.
[19]	NEJAD S M, VELDHUIS S L, RICHARD G, et al.Selection of single chain variable fragment (sc Fv) antibodies from a hyperimmunized phage display library for the detection of the antibiotic monensin[J].Journal of Immunological Methods, 2010, 360 (1-2) :103-118.
[20]	孟辉, 温凯, 沈建忠, 等.抗庆大霉素噬菌体抗体库的构建和筛选[J].中国畜牧兽医, 2011, 38 (1) :76-80.
[21]	NAGUMO Y, OGURI H, TSUMOTO K, et al.Phage-display selection of antibodies to the left end of CTX3C using synthetic fragments[J].Journal of Immunological Methods, 2004, 289 (1-2) :137-146.
[22]	ZHAO S W, SHEN P P, ZHOU Y, et al.Selecting peptide ligands of microcystin-LR from phage displayed random libraries[J].Environment International, 2005, 31 (4) :535-541.
[23]	SHAW I, O’REILLY A, MARGARET C, et al.Development of a high-affinity anti-domoic acid sheep sc Fv and its use in detection of the toxin in shellfish[J].Analytical Chemistry, 2008, 80 (9) :3205-3212.
[24]	DOYLE P J, GHAHROUDI M-A, GAUDETTE N, et al.Cloning, expression, and characterization of a single-domain antibody fragment with affinity for 15-acetyl-deoxynivalenol[J].Molecular Immunology, 2008, 45 (14) :3703-3713.
[25]	HOUWELINGEN A V, SAEGER S D, RUSANOVA T, et al.Generation of recombinant alpaca VHH antibody fragments for the detection of the mycotoxin ochratoxin A[J].World Mycotoxin Journal, 2008, 1 (4) :407-417.
[26]	WANG Y, WANG H, LI P, et al.Phage-displayed peptides that mimic aflatoxins and its application in immunoassay[J].Journal of Agricultural and Food Chemistry, 2013, 61 (10) :2426-2433.
[27]	XUE S, LI H P, ZHANG J B, et al.Chicken single-chain antibody fused to alkaline phosphatase detects Aspergillus pathogens and their presence in natural samples by direct sandwich enzyme-linked immunosorbent assay[J].Analytical Chemistry, 2013, 85 (22) :10992-10999.
[28]	QIU Y L, HE Q H, XU Y, et al.Deoxynivalenol-mimic nanobody isolated from a na6ve phage display nanobody library and its application in immunoassay[J].Analytica Chimica Acta, 2015, 887:201-208.
[29]	HU Z Q, LI H P, WU P, et al.An affinity improved singlechain antibody from phage display of a library derived from monoclonal antibodies detects fumonisins by immunoassay[J].Analytica Chimica Acta, 2015, 867:74-82.
[30]	XU Y, XIONG L, LI Y, et al.Citrinin detection using phagedisplayed anti-idiotypic single-domain antibody for antigen mimicry[J].Food Chemistry, 2015, 177:97-101.
[31]	WANG X, HE Q, YANG X, et al.Anti-idiotypic VHH phage display-mediated immuno-PCR for ultrasensitive determination of mycotoxin zearalenone in cereals[J].Talanta, 2016, 147:410-415.
[32]	GUPTA A K, SINGH A.Single-domain antibody selected from the phage display library neutralizes Escherichia coli endotoxin-induced effects on leukocytes in vitro and in Swiss albino mice[J].International Journal of Infectious Diseases, 2016, 45:136-137.
[33]	HUA X D, SHI H Y, WANG M H.Phage display peptide library technology and its research progress in immunoassay of pesticide residue[J].Journal of Food Safety and Quality, 2014 (12) :3955-3961.
[34]	LI T, ZHANG Q, LIU Y, et al.Production of recombinant Sc Fv antibodies against methamidophos from a phage-display library of a hyperimmunized mouse[J].Journal of Agricultural and Food Chemistry, 2006, 54 (54) :9085-9091.
[35]	BRICHTA J, VESELA H, FRANEK M.Production of sc Fv recombinant fragments against 2, 4-dichlorophenoxyacetic acid hapten using na6ve phage library[J].VeterinárníMedicína, 2003, 48 (9) :237-247.
[36]	LI Y, COCKBURN W, KILPATRICK J B, et al.High affinity Sc Fvs from a single rabbit immunized with multiple haptens[J].Biochemical and Biophysical Research Communications, 2000, 268 (2) :398-404.
[37]	GONZáLEZ-TECHERA A, ZON M A, MOLINA P G, et al.Development of a highly sensitive noncompetitive electrochemical immunosensor for the detection of atrazine by phage antiimmunocomplex assay[J].Biosensors and Bioelectronics, 2015, 64 (64) :650-656.
[38]	LIU Z, LIU J, WANG K, et al.Selection of phage-displayed peptides for the detection of imidacloprid in water and soil[J].Analytical Biochemistry, 2015, 485 (23) :365-367.
[39]	HUA X, LIU X, SHI H, et al.Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide[J].RSC Advances, 2014, 4 (80) :42445-42453.
[40]	LEE J, KIM Y H, MIN J.Screening of phage display-derived peptides to detect parabens[J].New Biotechnology, 2016, 33:172-173.
[41]	PINI A, GIULIANI A, FALCIANI C, et al.Antimicrobial activity of novel dendrimeric peptides obtained by phage display selection and rational modification[J].Antimicrobial Agents and Chemotherapy, 2005, 49 (7) :2665-2672.
[42]	BISHOP-HURLEY S L, REA P J, MCSWEENEY C S.Phage-displayed peptides selected for binding to Campylobacter jejuni are antimicrobial[J].Protein Engineering Design and Selection, 2010, 23 (10) :751-757.
[43]	RAO S S, MOHAN K V, ATREYA C D.A peptide derived from phage display library exhibits antibacterial activity against E.coli and Pseudomonas aeruginosa[J].Plos One, 2013, 8 (2) :e56081.
[44]	TAN Y, TIAN T, LIU W, et al.Advance in phage display technology for bioanalysis[J].Biotechnology Journal, 2016, 11 (6) :732-745.
[45]	AGRAWAL S, KULABHUSAN P K, JOSHI M, et al.A high affinity phage-displayed peptide as a recognition probe for the detection of Salmonella typhimurium[J].Journal of Biotechnology, 2016, 231:40-45.

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噬菌体展示技术在食品安全分析中的应用

作者简介: 赵岩岩 (1987-) , 女, 博士, 讲师, 研究方向:微生物与生物技术, E-mail:zhaoyanyan@hist.edu.cn。; 屠康 (1968-) , 男, 博士, 教授, 研究方向:农产品无损检测、农产品贮藏与加工, E-mail:kangtu@njau.edu.cn。;

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Application of phage display technology in food safety

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作者简介:
赵岩岩 (1987-) , 女, 博士, 讲师, 研究方向:微生物与生物技术, E-mail:zhaoyanyan@hist.edu.cn。;

屠康 (1968-) , 男, 博士, 教授, 研究方向:农产品无损检测、农产品贮藏与加工, E-mail:kangtu@njau.edu.cn。;