Separation and purification of isochlorogenic acid A from Artemisiae argyi folium by high- speed counter- current chromatography
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摘要: 应用制备型高速逆流色谱法（HSCCC）分离纯化艾叶中的异绿原酸A。选用弱极性溶剂体系正己烷-乙酸乙酯-乙醇-水（3∶7∶4∶6,V/V）为两相溶剂系统,上相为固定相,下相为流动相,在流速2 m L/min,转速900 r/min,检测波长280 nm的条件下对粗提物进行制备分离,一次进样25 mg,可得到11 mg样品。利用高效液相色谱（HPLC）检测样品的纯度为99.01%。将得到的样品进行高效液相-离子阱-飞行时间质谱（LCMS-IT-TOF）、核磁共振氢谱（1H-NMR）检测确定其化学结构,并确定该样品为异绿原酸A。实验表明制备型高速逆流色谱可以成功地将艾叶中的异绿原酸A进行分离纯化,该方法分离效率高,对异绿原酸A在食品医疗领域的应用具有重要意义。Abstract: Isochlorogenic acid A was separated and purified from Artemisiae argyi folium by preparative high-speed counter- current chromatography( HSCCC) using a weak polarity solvent system consisting of n- hexaneethyl acetate- ethanol- water( 3∶ 7∶ 4∶ 6,v / v / v / v),the upper phase acted as stationary phase and the lower phase served as mobile phase.The separation process was performed at a flow rate of 2 m L / min,the rotation rate of the apparatus was set at 900 r / min,and the effluents were detected at 280 nm,a total of 11 mg of target compound was obtained in one step separation from 25 mg of crude extract.The results from HPLC analysis showed that the compound with 99.01% purity and the chemical structure was characterized as Isochlorogenic A by high performance liquid chromatography- mass spectrum( LCMS- IT- TOF) and Nuclear Magnetic Resonance Spectroscopy(1H- NMR).It showed that preparative high- speed counter- current chromatography could separate and purify Isochlorogenic acid A successfully from Artemisiae argyi folium. The method was of high separation efficiency,and great importance for isochlorogenic acid A applications in the food and medical fields.
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