乳酸菌肽聚糖吸附丙烯酰胺的构效关系研究

白红雨; 刘清波; 崔蔚然; 唐祯玥; 张雨松; 邵美丽

doi:10.13386/j.issn1002-0306.2024040136

乳酸菌肽聚糖吸附丙烯酰胺的构效关系研究

Structure-Activity Relationship of Acrylamide Adsorption by Peptidoglycan of Lactic Acid Bacteria

摘要

摘要: 以5株乳酸菌（植物乳杆菌ATCC8014、植物乳杆菌806、植物乳杆菌1.0665、干酪乳杆菌ATCC393、嗜酸乳杆菌KLDS1.0307）肽聚糖（Peptidoglycan，PG）为研究对象，立足PG化学结构、PG微观结构、PG表面结构分别进行解析，分析比较PG结构与其吸附丙烯酰胺（Acrylamide，AA）的关系。结果表明，植物乳杆菌ATCC8014 PG对AA的吸附率最高，为89.93%；化学结构分析表明，随着PG的氨基己糖含量、糖链长度、谷氨酸含量的增加，其对AA的吸附能力由56.76%提升至89.93%；天冬氨酸作为肽桥较丝氨酸更有利于PG吸附AA，吸附率最大提升量为33.17%；随着PG乙酰化程度的增大，对AA的吸附率分别降低了29.62%、34.00%、41.96%、69.03%；微观结构表明，PG的比表面积、孔容大小与吸附率呈正相关，植物乳杆菌ATCC8014 PG的比表面积、孔容最大，分别为0.9857 m²/g、0.0056 cm³/g，对AA吸附率最高，为89.93%；PG的孔径大小与吸附率呈负相关，嗜酸乳杆菌KLDS1.0307 PG孔径最大，为49.2072 nm，对AA吸附率最低，为56.76%；表面结构分析表明，PG的粗糙度与其吸附AA的能力无关。上述结果表明，不同PG的结构差异会造成对AA吸附能力的差异，本研究可为乳酸菌PG生物脱毒机理研究提供参考。

Abstract: This research investigated the peptidoglycan (PG) extracted from five strains of lactic acid bacteria (Lactobacillus plantarum ATCC8014, Lactobacillus plantarum 806, Lactobacillus plantarum 1.0665, Lactobacillus casei ATCC393 and Lactobacillus acidophilus KLDS1.0307), focusing on the correlation between PG's structural configurations and its ability to adsorb acrylamide (AA). A comprehensive analysis was conducted to examine the chemical, microscopic, and surface structures of PG. The findings showed that PG from Lactobacillus plantarum ATCC8014 possessed the superior AA adsorption rate of 89.93%. Chemical structure analysis indicated that increments in the PG's hexosamine content, sugar chain length, and glutamic acid content were significantly and positively linked to an increased AA adsorption capacity, with the rate escalated from 56.76% to 89.93%. The utilization of aspartic acid as a peptide bridge was identified as more effective for AA adsorption by PG than serine, achieving the highest adsorption rate increment of 33.17%. Conversely, heightened PG acetylation was associated with a diminished AA adsorption rate, with respective declines of 29.62%, 34.00%, 41.96%, and 69.03%. Microstructural evaluation revealed a positive correlation between the specific surface area and pore volume of PG and the adsorption rate. Lactobacillus plantarum ATCC8014 PG exhibited the most considerable specific surface area (0.9857 m²/g) and pore volume (0.0056 cm³/g), which corresponded to its highest AA adsorption rate of 89.93%. Conversely, Lactobacillus acidophilus KLDS1.0307 PG, with the largest pore size (49.2072 nm), presented the lowest AA adsorption rate of 56.76%, indicating a negative correlation. The surface structure analysis recommended that the roughness of PG did not significantly influence its ability to adsorb AA. The study's conclusions underscore the influence of PG structural diversity on AA adsorption efficacy, offering references for advancing the understanding of biological detoxification mechanisms in lactic acid bacteria PG.