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中国精品科技期刊2020
马可心,相海峤,于潇,等. 柑橘纤维与菊粉添加量对羊肉蛋白结构及肉糜品质的影响[J]. 宝威体育平台,2025,46(8):1−9. doi: 10.13386/j.issn1002-0306.2024040084.
引用本文: 马可心,相海峤,于潇,等. 柑橘纤维与菊粉添加量对羊肉蛋白结构及肉糜品质的影响[J]. 宝威体育平台,2025,46(8):1−9. doi: 10.13386/j.issn1002-0306.2024040084.
MA Kexin, XIANG Haiqiao, YU Xiao, et al. Effects of Citrus Fiber and Inulin Addition on the Lamb Protein Structure and Mince Quality[J]. Science and Technology of Food Industry, 2025, 46(8): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024040084.
Citation: MA Kexin, XIANG Haiqiao, YU Xiao, et al. Effects of Citrus Fiber and Inulin Addition on the Lamb Protein Structure and Mince Quality[J]. Science and Technology of Food Industry, 2025, 46(8): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024040084.

柑橘纤维与菊粉添加量对羊肉蛋白结构及肉糜品质的影响

Effects of Citrus Fiber and Inulin Addition on the Lamb Protein Structure and Mince Quality

  • 摘要: 为了探究柑橘纤维与菊粉对羊肉蛋白结构及肉糜品质的影响。本文分别考察了柑橘纤维(0.5%、1.0%、1.5%、2.0%)与菊粉(2.0%、3.0%、4.0%、5.0%)的添加对羊肉蛋白质构象、水分分布、质构和微观结构等指标的影响。结果表明:添加2.0%柑橘纤维显著(P<0.05)提高了二硫键含量(增加12%)、疏水相互作用(增加14%)、并增加了蛋白质β-折叠含量(增量8.14%)和无规则卷曲含量(增量1.29%)。添加2.0%柑橘纤维使蛋白质内部结构发生交联,提高了不易流动水的相对含量,促进形成有序、致密、连续的蛋白质三维网络结构,显著提升了肉糜的保水性和硬度(P<0.05)。当菊粉添加量为3.0%时,蛋白质的β-折叠含量显著增加了8.68%(P<0.05),无规则卷曲含量增加了0.31%,显著提高了肉糜的保水性。然而,与柑橘纤维不同的是,3.0%的菊粉添加,促进了色氨酸残基的暴露且对二硫键以及酪氨酸残基无显著影响(P>0.05)。综上,2.0%柑橘纤维与3.0%菊粉均可不同程度地修饰蛋白质结构,有效地提高肉糜的保水性、改善肉制品品质。

     

    Abstract: In this study, the effects of adding citrus fiber (0.5%, 1.0%, 1.5%, and 2.0%) and inulin (2.0%, 3.0%, 4.0%, and 5.0%) were investigated on the lamb mince quality, water distribution, texture, and the protein conformation and the microstructure of lamb protein. Results showed that adding 2.0% citrus fiber significantly increased the disulfide bond content (by 12%) and hydrophobic interactions (by 14%), along with increments in the β-folding content (increment of 8.14%) and irregular curling content (increment of 1.29%). Adding 2.0% citrus fiber promotes cross-linking of proteins increases the relative content of water that was immoblilized water, promotes the formation of an ordered, dense, and continuous three-dimensional protein network structure, and significantly improved the water retention and hardness of minced meat (P<0.05). The addition of 3.0% inulin also significantly increased the β-folding content of the protein by 8.68% (P<0.05), increased the irregular curling content by 0.31%, and the water retention is significantly improved. However, unlike citrus fibers, 3.0% inulin addition promoted the exposure of tryptophan residues and had no significant effect on disulfide bonds and tyrosine residues (P>0.05). In summary, either 2.0% citrus fiber or 3.0% inulin could friendly modify the protein structure, and effectively improve the water retention of minced meat and the quality of meat products.

     

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