Breeding of High Performance Metarhizium anisopliae Strain by ARTP/UV Mutagenesis
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摘要: 为获得高性能的金龟子绿僵菌菌株，本研究采用常压室温等离子体（Atmospheric and room temperature plasma，ARTP）和紫外线复合诱变处理金龟子绿僵菌，通过比较产孢快慢和生长直径大小进行初筛，用抗紫外能力和毒力作为复筛指标选育菌株，并对育种获得的突变株进行耐热性和遗传稳定性试验，最终在ARTP 40 s和紫外120 s时筛选出一株产孢量高、耐紫外线、毒力强、遗传稳定的优良菌株AU34。该菌株产孢时间比原始菌株快，产孢量为1.63±0.22（108 cell/cm2），较原始菌株提高了63.81%。经紫外照射5 min后，AU34存活率为3.18%，比原始菌株更耐紫外照射。并且AU34对小菜蛾的毒力强，校正死亡率为85.71%，半数致死时间（Half-lethal time，LT50）为6.12 d。与原始菌株相比，AU34的耐热性也有较大提高。传代培养6代后，菌株AU34的产孢量无明显变化，具有良好的遗传稳定性。Abstract: After atmospheric and room temperature plasma(ARTP)and ultraviolet(UV)compound mutation,a high-performance strains of Metarhizium Anisopliae was selected. By comparing the speed of sporulation and the size of growth diameter,selected with the ability of anti-ultraviolet and virulence as the re-screening index,and tested for heat resistance and genetic stability,a good strain AU34 with high sporulation,UV tolerance,strong virulence and stable genetic was selected at the time of ARTP 40 s and UV 120 s. The sporulation time of the strain was faster than the original strain,and the sporulation amount was 1.63±0.22(108 cell/cm2),which was 63.81% higher than the original strain. After 5 min of UV irradiation,the survival rate of AU34 was 3.18%,which was more resistant to UV irradiation than the original strain. The toxicity of AU34 to Plutella xylostella L. was high. The adjusted mortality rate was 85.71% and LT50 was 6.12 days. Compared with the original strain,the heat resistance of AU34 was also improved. After subculture for 6 generations,the sporulation of strain AU34 had no obvious change and had good genetic stability.
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