Extraction Technology Optimization and Antioxidant Activity of Potato Crude Protein in Vitro
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摘要: 目的：为充分利用马铃薯中的粗蛋白资源，减少资源的浪费和环境污染，优化了马铃薯粗蛋白工艺。方法：采用单因素试验结合响应面法，以马铃薯的粉碎粒度、提取时间、料液比为自变量，以马铃薯粗蛋白含量为响应值，得到马铃薯粗蛋白的优选提取工艺。结果：马铃薯粗蛋白的最佳提取工艺为粒度80目，提取时间4 h，料液比1：25 g/mL，此时得到马铃薯粗蛋白的含量为0.27 mg/g，与预测值0.28 mg/g接近。相对误差为2.56%。体外抗氧化实验表明，马铃薯粗蛋白对ABTS+·（2，2-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐）、DPPH·（1，1-二苯基-2苦肼基）、·OH清除能力较强，当浓度为1 mg/mL时，其对ABTS+·清除率高达92.54%。结论：此工艺稳定可靠，可用于实际工业生产中马铃薯粗蛋白含量的提取，为进一步开发利用马铃薯粗蛋白提供理论依据。
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关键词:
- 马铃薯 /
- 粗蛋白 /
- Box-Behnken响应面法 /
- 提取工艺优化 /
- 体外抗氧化活性
Abstract: Objection:In order to make full use of potato protein resources and reduce the waste of potato protein resources and environmental pollution,the optimum extraction process of potato protein was optimized. Method:The optimum extraction technology of potato protein was obtained by single factor experiment combined with response surface methodology,taking particle size,extraction time,solid-liquid ratio as independent variables and potato protein content as response value. Results:The optimum extraction technology of potato protein was 80 meshes in diameter,4 hours in extraction time and 1:25 g/mL in solid-liquid ratio. At this time,the content of potato protein was 0.27 mg/g,which was closed to the predicted value 0.28 mg/g. The relative error was 2.56%. In vitro antioxidant experiments showed that potato protein had a strong ability to scavenge ABTS+·,DPPH,·OH.When the concentration was 1 mg/mL,the scavenging rate of potato protein to ABTS reached 92.54%. Conclusion:This process was stable and reliable,and could be used to optimize the extraction of potato protein content in actual industrial production. It would provide a theoretical basis for further development and utilization of potato protein. -
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