Process Optimization of Soluble Antioxidant Dietary Fiber Extracted from Spongy Layer of Guanxi Honey-pomelo Peels by Ultrasonic Assisted Enzymatic Method
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摘要: 研究琯溪柚皮海绵层水溶性膳食纤维（SADF）的最佳提取工艺，实现蜜柚废弃物综合利用，减少资源浪费。以平和琯溪蜜柚柚皮为原料，采用超声波辅助酶法提取SADF。在单因素实验考察超声波作用时间、料液比、纤维素酶添加量、纤维素酶作用温度和酶作用时间对柚皮SADF的得率和羟自由基清除率影响的基础之上，进行3因素3水平Box-Benhnken双响应面结合Matlab优化实验，确定最佳提取方案为：超声波前处理30 min，料液比1：55 g/mL （1：53~1：55 g/mL），纤维素酶添加量3%，纤维素酶作用温度50℃（48~50℃），纤维素酶作用时间90 min （上限值C=90 min）。此时，柚皮SADF的得率理论值可以达到31.40%（25.12%~31.40%），羟自由基清除率理论值可达到66.16%（51.50%~66.16%），与实际值（得率：32.82%±0.33%，清除率64.43%±1.88%）差异不显著（P>0.05）。可见，Box-Benhnken结合Matlab优化工艺条件下提得的蜜柚海绵层SADF同时具有较好的得率与羟自由基清除率，为功能性原料提取应用提供了新的思路。
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关键词:
- 琯溪蜜柚 /
- 柚皮海绵层 /
- 水溶性抗氧化膳食纤维 /
- 超声波辅助酶法 /
- Box-Benhnken结合Matlab优化
Abstract: In this study,the optimal extraction process of soluble antioxidant dietary fiber(SADF)from sponge layer of Guanxipomelo peels was studied to realize comprehensive utilization of honey-pomelo waste and reduce resource waste.Guanxi honey-pomelo peels were selected as raw material,and the SADF from the spongy layer of the peels was extracted by ultrasonic-assistedenzymatic method. Based on the single factor experiment which investigated the influence of ultrasonic action time,solid-liquid ratio,cellulase dosage,cellulase temperature,as well as cellulase action time on both extraction yield and antioxidant activity of SADF,Box-Benhnken dual-response surface optimization experiment characterized with 3 levels and 3 factors,combined with Matlabanalysis was carried out. The optimal technological condition was determined as follows: ultrasonic pre-treatment for 30 min,solid-liquid ratio of 1∶55 g/mL(1∶53~1∶55 g/mL),cellulase dosage of 3%,cellulase action temperature of 50 ℃(48~50 ℃),cellulase action time of 90 min(The upper limit C=90 min). On this condition,the theoretical extraction yield and hydroxyl radical clearance rate of SADF from Guanxi honey-pomelo peels can reach 31.40%(25.12%~31.40%)and 66.16%(51.50%~66.16%),respectively,which showed no significant difference between the actual value(extraction yield: 32.82%±0.33%,clearance rate: 64.43%±1.88%)(P>0.05). It can be seen that the SADF of the sponge layer from pomelo peels obtained under the optimized process conditions by Box-Benhnken combined with Matlab was of better extraction yield and hydroxyl radical clearance rate,so that providing a new idea for the extraction and application of functional raw materials. -
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