Comparison of Physical Degradation Techniques of Aflatoxin B1 in Guizhou Tartary Buckwheat Rice
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摘要: 应用微波、紫外线、γ射线辐照三种物理技术降解苦荞饭中黄曲霉毒素B1(AFB1),采用高效液相色谱法测定AFB1的含量。试验考察了微波功率、微波时间、紫外照射时间、紫外照射高度、γ射线辐照剂量对其降解效率的影响。结果表明,苦荞饭中AFB1降解率依次为γ射线辐照 > 紫外降解 > 微波降解,γ射线在辐照剂量为20 kGy时,降解率分别为33.6%±1.5%(污染剂量7.5 μg/kg)、35.1%±1.7%(污染剂量30 μg/kg)、45.7%±1.3%(污染剂量60 μg/kg)。与单独降解技术相比,将任意两种或者三种降解技术联合后,其降解率均显著增加(P<0.05),微波+紫外线+γ射线辐照三种技术联合降解率可达51.9%±0.9%(污染剂量7.5 μg/kg)、66.5%±0.7%(污染剂量30 μg/kg)、71.5%±0.9%(污染剂量60 μg/kg)。三种物理降解技术对于苦荞饭中低剂量的AFB1污染有一定的应用价值。Abstract: Aflatoxin B1(AFB1)contents in tartary buckwheat rice was estimated by HPLC after degradation by microwave,ultraviolet and γ ray irradiation. The microwave power,microwave time,ultraviolet irradiation time,ultraviolet irradiation height and γ radiation dose were investigated. The results showed that:The degradation rate of AFB1 in tartary buckwheat rice was gamma irradiation > UV degradation > microwave degradation. When the radiation dose was 20 kGy,The degradation rate were 33.6%±1.5%(pollution dose of 7.5 μg/kg),35.1%±1.7%(pollution dose of 30 μg/kg),and 45.7%±1.3%(pollution dose of 60 μg/kg),respectively. Compared to separate degradation technologies,after combining any two or three degradation technologies,the degradation rate was significantly increased compared to the single degradation technique(P<0.05).The combined degradation rates of the three technologies of microwave,ultraviolet and γ ray irradiation reached 51.9%±0.9%(pollution dose of 7.5 μg/kg),66.5%±0.7%(pollution dose of 30 μg/kg),and 71.5%±0.9%(pollution dose of 60 μg/kg),respectively. Three kinds of physical degradation technologies would have certainly application value for low-dose AFB1 pollution in tartary buckwheat rice.
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Keywords:
- tartary buckwheat rice /
- aflatoxin B1 /
- microwave /
- ultraviolet /
- gamma irradiation /
- degradation
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