Optimization of Extraction Technology of Visceral Metallothionein from Patinopecten yessoensis
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摘要: 本文主要探究虾夷扇贝内脏中金属硫蛋白的提取方法。以提取液中金属硫蛋白含量为评价指标,用单因素和响应面试验优化提取剂浓度,提取剂pH及加热温度。粗提物经分子筛预装柱分离纯化。结果发现,虾夷扇贝内脏中金属硫蛋白最佳提取条件为:Tris-HCl浓度0.01 mol/L,Tris-HCl pH为8.23,加热温度为70 ℃。在此条件下实际提取液中MT含量为0.115 mg/g。分子筛柱层析一步纯化后获得虾夷扇贝内脏源金属硫蛋白MT-I和MT-II 2种异构体的混合物,纯度为81%。本研究获得的提取工艺可得到较高纯度的虾夷扇贝内脏源金属硫蛋白。Abstract: This paper mainly discussed the extraction of metalothionein from the viscera of Patinopecten yessoensis. Based on the content of metallothionein in the extract, the concentration of extracting agent, pH and heating temperature were optimized by single factor experiment and response surface experiment.The molecular sieve pre-packed column was used to separated the crude extra-ction. The results showed that the optimum extraction conditions of metallothi-onein in the viscera of Patinopecten yessoensis were as follows:the concentrate-ion of extractant was 0.01 mol/L, the pH of extractant was 8.23, and the heating temperature was 70℃. Under these conditions, the content of MT ext-racted was 0.115 mg/g. The mixture of MT-I and MT-II two isomers were purified by molecular sieve column chromatography. The purity of the MT-I and MT-II mixture were 81%. The extraction technology obtained in this study could obtain high purity of visceral metallothionein from Patinopecten yessoensis.
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