双波长法测定南瓜中直链与支链淀粉含量

李晓娟; 李柱刚; 王珣; 韩俊岩; 赵伟; 赵日; 陆杰

doi:10.13386/j.issn1002-0306.2018.08.042

双波长法测定南瓜中直链与支链淀粉含量

李晓娟¹,
李柱刚^2, ,,
王珣¹,
韩俊岩¹,
赵伟¹,
赵日¹,
陆杰³

1. 黑龙江省农业科学院博士后科研工作站, 黑龙江省农业科学院生物技术研究所, 黑龙江省作物与家畜分子育种重点实验室, 黑龙江哈尔滨 150086;
2. 黑龙江省农业科学院耕作栽培研究所, 黑龙江哈尔滨 150086;
3. 黑龙江省农业科学院齐齐哈尔分院, 黑龙江齐齐哈尔 161041

基金项目:

公益性行业(农业)科研专项经费项目(201303112)

国家自然科学基金(31471546)。

详细信息

作者简介:
李晓娟(1982-),女,博士,助理研究员,研究方向:南瓜资源开发与利用,E-mail:xiaojuanli_2011@163.com。

通讯作者:
李柱刚(1972-),男,博士,研究员,研究方向:南瓜育种及其活性物质分析与利用,E-mail:lizhugang@163.com。

中图分类号: TS255.7
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出版历程
- 收稿日期: 2017-07-31
- 网络出版日期: 2020-11-23
- 刊出日期: 2018-04-14

Determination of amylose and amylopectin in pumpkin with dual-wavelength colorimetric method

1. Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang, Biotechnology Research Institute, Heilongjiang Academy of Agricultural Sciences, Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin 150086, China;
2. Institute of Tillage and Cultivation, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;
3. Qiqihar Swb-academy of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161041, China

摘要

摘要: 为建立同时测定南瓜中直链和支链淀粉的双波长比色法。本文采用正丁醇结晶法分离纯化南瓜中直链和支链淀粉并测定其含量。结果表明,分离纯化得到的直链和支链淀粉蓝值分别为0.874和0.196;根据双波长原理和扫描谱图,确定其测定波长为645、565 nm,参比波长为495、730 nm处测定南瓜中直链与支链淀粉含量;南瓜样品中直链淀粉占21.64%,支链淀粉占50.19%,总淀粉含量为71.83%,相对标准偏差分别为0.97%和1.06%,平均回收率分别为98.62%和99.42%,检出限分别为0.0116和0.0644 mg/mL。正丁醇结晶法能有效地分离南瓜直链与支链淀粉,双波长法可同时获得南瓜中3个淀粉含量指标,该方法既节省了样品,又提高了效率。同时,该方法的建立也为南瓜淀粉理化特性的研究提供了技术支持。
- 南瓜 /
- 直链淀粉 /
- 支链淀粉 /
- 提纯 /
- 双波长比色法
Abstract: Dual-wavelength spectrophotometry was applied to the determination of amylose and amylopectin from pumpkin. N-butanol crystallization separation and purification of pumpkin in the linear and amylopectin,and content of them were mensurated. The results showed that the blue values of linear and amylopectin isolated and purified were 0.874 and 0.196,respectively. According to the principle of dual-wavelength spectrophotometry and scanning spectra,the contents of amylose and amylopectin in pumpkin were determined at the selected wavelengths of 645 and 565 nm with respective reference wavelengths of 495 and 730 nm. The amylose in pumpkin samples accounted for 21.64%,the amylopectin was 50.19% and the total starch was 71.83%. The RSD was 0.97% and 1.06%,respectively. The average recovery of amylose and amylopectin were 98.62% and 99.42%,respectively,while detection limit were 0.0116 and 0.0644 mg/mL. N-butanol crystallization method could separate and purify of amylose and amylopectin in pumpkin effectively. The detection method could obtain three kinds of starch content simultaneously. The developed dual-wavelength colorimetric method was not only cost-saving and efficient for determination of amylose and amylopectin,but also provide a basis for research on physicochemical properties of starch in pumpkin.
- pumpkin /
- amylase /
- amylopectin /
- purification /
- dual-wavelength colorimetric method