摘要:
目的:以干蒲公英茎叶为原料,优化其绿原酸提取工艺。方法:采用酶解超声组合法,以绿原酸得率为指标,在单因素实验的基础上,确定酶添加量、酶解时间、超声功率和超声时间为主要影响因素及其水平值,采用Box-Behnken法设计四因素三水平实验进行响应面优化,并建立二次多项数学模型。结果:蒲公英中绿原酸的最佳提取工艺条件:纤维素酶添加量0.3%(占干料的百分比)、酶解时间1.0 h、酶解温度50 ℃、酶解pH4、超声功率163 W、超声时间1.7 h、料液比1:20 g/mL。在此条件下,绿原酸平均得率为2.14%±0.02%,与模型预测值(2.12%)相近。结论:酶解超声组合方法较适合提取蒲公英中的绿原酸,响应面分析法较好地优化了提取工艺。
关键词:
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蒲公英
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绿原酸
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纤维素酶
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超声波
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提取
Abstract:
Objective:To optimize the technology of extracting chlorogenic acid from the stem and leaf of dandelion. Method:Using the method of enzymatic ultrasonic combination,cellulase additive amount,enzymolysis time,ultrasonic power and ultrasonic time were chose as the main factors.Based on the single experiments,the extracting processing parameters of chlorogenic acid in dandelion were optimized by response surface method,and the multivariate two regression model was established by the index of the chlorogenic acid yield. Results:The optimum extracting parameters were determined as follows:Cellulase additive amount 0.3%(percentage of dry material),enzymolysis time 1.0 h,enzymolysis temperature 50 ℃,enzymolysis pH4,ultrasonic power 163 W,ultrasonic time 1.7 h,solid-liquid ratio 1:20 g/mL. On this extracting condition,the yield of chlorogenic acid was 2.14%±0.02%,it was similar to the model predictive value 2.12%. Conclusions:The method of enzymatic ultrasonic combination is suitable to extract chlorogenic acid from dandelion,and the extracting technology can be well optimized by response surface method.
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