Advance in RPA detection technologies of foodborne pathogenic bacteria
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摘要: 食源性致病菌是食品安全的重要隐患,开发针对食源性致病菌快速、有效的检测技术迫在眉睫。在诸多食源性致病菌检测手段中,重组酶聚合酶扩增技术(RPA),因具有比传统分子及免疫学检测技术如聚合酶链式反应(PCR)、实时荧光定量PCR、酶联免疫吸附技术(ELISA)等更快速、灵敏、特异、简便以及实用性强等方面优势,现已在一定程度上得到应用。本文将对RPA技术原理及其衍生技术包括直接重组酶聚合酶扩增技术(Direct-RPA)、重组酶聚合酶扩增侧流层析技术(RPA-LFD)、重组酶聚合酶扩增酶联免疫吸附技术(RPA-ELISA)、实时重组酶聚合酶扩增技术(Real-time RPA)、RPA微流体等技术进行简要综述。Abstract: Food-borne pathogens are an important risk of food safety and thus the development of rapid and effective detection technology aimed at food-borne pathogens is imminent.Among many detection methods for foodborne pathogens,recombinase polymerase amplification(RPA) has been to some extent applied due to many advantages such as faster,simpler,more sensitive and practical compared to traditional molecular and immunological detection techniques such as polymerase chain reaction (PCR),real-time quantitative PCR, enzyme-linked immunosorbent assay (ELISA). In this review, the principle of RPA technology and its derivative technologies including direct recombinase polymerase amplification(Direct-RPA),RPA-lateral flow dipstick(RPA-LFD), RPA-enzyme-linked immunosorbent assay (RPA-ELISA), real-time recombinase polymerase amplification(Real-time RPA) and RPA microfluidic were briefly reviewed.
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Keywords:
- food borne pathogens /
- RPA /
- food safety /
- detection technology
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