棘孢木霉液体发酵条件优化

宋巧英; 朱振元

doi:10.13386/j.issn1002-0306.2018.06.026

棘孢木霉液体发酵条件优化

宋巧英,
朱振元^,

食品营养与安全教育部重点实验室, 天津科技大学食品工程与生物技术学院, 天津 300457

基金项目:

国家星火计划重点项目(2015GA610001)。

详细信息

作者简介:
宋巧英(1993-),女,博士,研究方向:天然生物材料的纯化、改性及保健功能,E-mail:969354025@qq.com。

通讯作者:
朱振元(1969-),男,博士,教授,研究方向:生物资源与功能食品,E-mail:zhyuanzhu@tust.edu.cn。

中图分类号: TS201.1
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出版历程
- 收稿日期: 2017-07-16
- 网络出版日期: 2020-11-23
- 刊出日期: 2018-03-14

Optimization of liquid fermentation conditions of Trichoderma asperellum

SONG Qiao-ying,
ZHU Zhen-yuan^,

Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China

摘要

摘要: 为研究棘孢木霉液体发酵最优条件,以菌丝体干重和孢子数为指标,通过单因素正交实验对棘孢木霉液体发酵培养基进行优化,并测定棘孢木霉的基本成分。实验结果表明最佳发酵条件为:黄豆粉0.25%,KH₂PO₄ 0.3%,MgSO₄ 0.15%,接种量8×10⁶个/mL,装瓶量100 mL/250 mL,蛋白胨1.35%,葡萄糖3.5%,转速160 r/min,温度25 ℃,自然光照,pH7。此发酵条件可使棘孢木霉干重量达到19.453 g/L,孢子数达到4.4812×10⁹个/mL。本研究为棘孢木霉的工业化生产降低成本,缩短发酵周期打下理论基础。
- 菌丝体 /
- 孢子数 /
- 棘孢木霉 /
- 液体培养基
Abstract: To study the optimum conditions for the fermentation of Trichoderma asperellum,the effects of dry weight and spores number on mycelia were optimized by single factor orthogonal experiment. Experimental result showed that the best medium was soy flour medium and the optimum conditions were as follows:0.25% of Soy flour,0.3% of KH₂PO₄,0.15% of MgSO₄,8×10⁶/mL of inoculationamount,100 mL/250 mL of bottling volume,1.35% of Peptone,3.5% of glucose,160 r/min of rotating speed,25 ℃,natural light,and primary pH7.The fermentation conditions can make the dry weight of Trichoderma viride reach 19.453 g/L,the number of spores reached 4.4812×10⁹/mL. For the mass production of Trichoderma asperellum reduced the cost,shorten the fermentation cycle,laying the theoretical basis for the industrial production of Trichoderma asperellum.
- mycelium /
- number of spores /
- Trichoderma asperellum /
- liquid medium