Antioxidant activity and hepatoprotective effects of polysaccharide from Crassostrea gigas internal organs against CCl4 induced hepatic injury in mice
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摘要: 目的:研究牡蛎内脏多糖（CGOP）的体外抗氧化活性及对CCl4所致小鼠肝损伤的影响作用。方法:通过测定DPPH自由基、羟基自由基、ABTS自由基清除效果及FRAP还原能力研究CGOP的体外抗氧化能力;通过测定各组小鼠血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）含量及肝组织匀浆中超氧化物歧化酶（SOD）、丙二醛（MDA）含量,观察小鼠肝组织切片形态学变化,判断CGOP对小鼠急性肝损伤的影响。结果:CGOP具有较好的清除DPPH自由基、ABTS自由基、羟基自由基效果及FRAP还原能力,并能显著降低小鼠的ALT、AST及MDA含量（p<0.05）,显著升高小鼠的SOD含量（p<0.01）,减轻CCl4对肝细胞的病理损伤。结论:CGOP具有保护CCl4急性肝损伤的作用,为更充分利用牡蛎资源提供了基础数据。Abstract: Objective: To investigate the protective effect of polysaccharide from Crassostrea gigas internal organs ( CGOP) on acute liver injury induced by CCl4 in mice.Methods: CGOP was analyzed effects of antioxidant action by scavenging DPPH free radical, hydroxyl free radical, ABTS radical and reduction potential. ALT, AST levels in serum and MDA, SOD levels in liver tissues were examined. In addition, pathologic changes were examined by HE staining. Results: CGOP showed antioxidant abilities.And acute hepatic injury protections of CGOP were demonstrated by the fact that increased in activity of liver AST and ALT caused by CCl4 of mice treated were significantly inhibited while the MDA content was significantly lowered and SOD activity enhanced as well.Histopathological changes induced by CCl4 were also significantly reduced.The results indicated that CCl4 acute liver damages could be inhibited by CGOP.It provides the basic data for making better use of oyster resources.
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