Analysis of the fragrance components of the fruit of Rosa roxbughii fermented with different yeasts
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摘要: 采用顶空固相微萃取和气相色谱-质谱联用（GC-MS）技术对5种不同酒曲发酵刺梨鲜果前后香气成分进行分析检测。从5种发酵液中检测鉴定香气成分共计88种,其中共有成分8种,分别为醇类1种（乙醇）,醚类1种（二甲硫醚）,醛类1种（乙醛）,酸类1种（辛酸）,烃类2种（十七烷、正十八烷）,酯类2种（乙酸乙酯、油酸乙酯）。刺梨鲜果（发酵前）香气成分主要为烃类和酸类,以罗勒烯和辛酸为主;发酵后5种刺梨发酵液中酸类、醛类、酯类物质组成存在差异,但均产生香味浓烈、持久的新香味物质苯乙醇,而鲜果中含量较高的罗勒烯未检出,辛酸含量减少。通过对比5种不同酒曲发酵刺梨鲜果后产生的香味物质的含量及种类变化,甜酒曲（S3）和高效酒曲（S2）作为发酵刺梨鲜果的酒曲较佳。Abstract: The fragrance components of the fruit of Rosa roxbughii and its fermentations with different yeasts were tested by headspace solid-phase microextraction ( HS-SPME) and GC-MS. 88 aromatic compounds in the fruit of Rosa roxbughii and its fermentations were identified which included eight mutual substances: alcohols ( ethanol) , ethers ( methyl sulfide) , aldehydes ( acetaldehyde) , acids ( octanoic acid) and two kinds of hydrocarbons ( heptadecane and octadecane) , respectively.Hydrocarbons and acids were the main volatile components in the fruit of R.roxbughii and the main components were ocimene and octanoic acid.Although there was a significant difference in the composition of acids, aldehydes and esters in five different fermentations, phenethyl alcohol, a compound with strong and lasting fragrance, was found in all five fermentations. Besides, ocimene which with the highest relative content before fermentation was not found and octanoic acid content declined.Compared with the changes of contents and types of the volatile components from the fermentations of Rosa roxbughii with five different yeasts, liqueur koji ( S3) and high-efficiency starter ( S2) were the best yeasts for the fermentation of the fruit of Rosa roxbughii.
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Keywords:
- fruit of Rosa roxbughii /
- fragrance components /
- yeast /
- fermentations
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[1] 曾芳芳, 罗自生.刺梨营养成分的研究进展[J].浙江农业科学, 2015, 56 (11) :1753-1757. [2] 张春妮, 周毓, 汪俊军.刺梨药理研究的新进展[J].医学研究生学报, 2005, 18 (11) :93-95. [3] 代甜甜, 李齐激, 南莹, 等.刺梨抗氧化活性部位的化学成分[J].中国实验方剂学杂志, 2015, 21 (21) :62-65. [4] 戴支凯, 余丽梅, 杨小生.刺梨提取物 (CL) 抗肿瘤作用[J].中国中药杂志, 2007, 32 (14) :1453-1457. [5] 唐婧, 苏迪, 徐小蓉, 等.基于宏基因组学的茅台酒酒曲细菌的多样性分析[J].贵州农业科学, 2014, 42 (11) :180-183. [6] 吕磊, 王腾飞, 汤丹丹, 等.芝麻香型白酒芽孢杆菌培养基的筛选及其产香分析[J].酿酒科技, 2014, 35 (9) :29-32. [7] 姚万春, 唐玉明, 任道群, 等.白酒曲药微生物分离培养基的选择研究[J].酿酒, 2011, 38 (3) :39-41. [8] 刘永衡, 华惠敏, 吴桂君, 等.果酒酵母选育及酵母对香气成分影响的研究进展[J].中国酿造, 2013, 32 (10) :5-8. [9] 李永波, 岳文, 邓功成, 等.甜酒曲中主要的微生物菌群分离及发酵优化[J].黔南民族师范学院学报, 2013, 33 (10) :102-105. [10] 吴琼燕, 林捷, 简佩雯, 等.甜酒药曲理化品质及糯米酒香气成分研究[J].食品工业, 2016, 38 (12) :130-134. [11] 李筝, 韩北忠, 陈晶瑜, 等.优良酿酒酵母菌的发酵性能研究[J].中国酿造, 2008, 196 (19) :10-12. [12] 郝莹, 王卫卫, 王莉娟, 等.陕北传统米酒曲中优势菌种的分离、纯化及鉴定[J].检验检疫学刊, 2010, 20 (2) :44-47. [13] 姚敏, 谭书明, 张少才, 等.刺梨干酒发酵前后有效成分变化[J].食品与发酵工业, 2014, 40 (10) :123-127. [14] 姚敏, 谭书明, 徐素云, 等.刺梨干酒与发酵原汁挥发性风味成分分析[J].中国酿造, 2014, 33 (8) :144-148. [15] 香料解析 (二十六) :罗勒烯[J].国内外香化信息, 2013 (11) :15-17. [16] 杨波, 杨光, 李代禧, 等.苯乙醇香精与β-环糊精包合物的制备工艺研究[J].食品工业科技, 2007, 28 (1) :210-212, 230. [17] Jiao F, Liu Q, Sun G F, et al.Floral fragrances of Hemerocallis L. (daylily) evaluated by headspace solid-phase microextraction with gas chromatography-mass spectrometry[J].The Journal of Horticultural Science and Biotechnology, 2016, 91 (6) :573-581.
[18] 曹雪丹, 李二虎, 方修贵, 等.蓝莓酒主发酵前后挥发性成分变化的GC-MS分析[J].食品与发酵工业, 2015, 41 (3) :179-184. [19] 校大伟, 成春雷, 张金, 等.苯乙醛合成研究进展[J].化学试剂, 2008, 30 (12) :899-902, 926. [20] 张玉玉, 宋弋, 李全宏.食品中糠醛和5-羟甲基糠醛的产生机理、含量检测及安全性评价研究进展[J].食品科学, 2012, 33 (5) :275-280. [21] 何义国, 赵兴秀, 邓静, 等.白酒中酯类化合物的稳定性研究[J].酿酒科技, 2012 (12) :34-36. [22] 延鑫.气相色谱法测定地方白酒中乙酸乙酯、杂醇油含量[J].北京联合大学学报:自然科学版, 2009, 23 (3) :53-56. [23] 张房宇.反相高效液相色谱法测定白酒中乙酸乙酯、乳酸乙酯和β-苯乙醇的含量[J].酿酒科技, 2008, 29 (11) :108-111.
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