Pelargonium from carmine radish inhibits migration and invasion of human gastric cancer
-
摘要: 主要探讨胭脂萝卜天竺葵素对人胃癌细胞（MKN45）迁移侵袭能力的影响及机制。采用不同浓度的天竺葵素处理MKN45细胞,用MTT法、流式细胞术、细胞划痕实验和TranswellTM小室实验分别检测细胞增殖、周期、迁移和侵袭能力变化,并用蛋白印迹法检测细胞黏附蛋白E-cadherin和N-cadherin的表达。结果表明,6μg/m L天竺葵素能显著抑制细胞的增殖,迁移和侵袭,并使细胞阻滞在S期。天竺葵素抑制细胞迁移和侵袭的机制可能是促进E-cadherin蛋白的表达和抑制N-cadherin蛋白的表达。Abstract: The mechanisms of carmine radish pelargonium inhabited gastric cancer cells ( MKN45) migration and invasion was investigated in the study.After different concentrations of pelargonium treatment, MTT assay, flow cytometry (FCM) , cell wound healing and TranswellTM assay were used to detect the cell proliferation, cell cycle, cell migration and invasion ability. Protein expression of E-cadherin and N-cadherin were measured with western blot.The results showed 6 μg/m L pelargonium inhibited MKN45 cell proliferation, migration and invasion remarkable, induced cell S phase arrest. E-cadherin upregulation and N-cadherin downregulation might be the major mechanisms of pelargonium inhibited cell migration and invasion.
-
Keywords:
- pelargonium /
- carmine radish /
- gastric cancer /
- migration and invasion
-
[1] 左婷婷, 郑荣寿, 曾红梅, 等.中国胃癌流行病学现状[J].中国肿瘤临床, 2017, 44 (1) :52-59. [2] 陈小捷, 阴文娅.植物中花青素提取方法探究[J].食品工业科技, 2013, 34 (2) :395-399. [3] Murthy K N C, Sultanpur C M, Rao R M, et a1.Natural molecules as tumourinhibitors:Promises and prospects[J].Journal of Herbal Medicine, 2014, 4 (4) :175-187.
[4] 许胜, 屈达才, 黄慧明, 等.花色素及其花色苷单体抗肿瘤活性研究进展[J].食品工业科技, 2016, 37 (14) :379-384. [5] Novotny J A, Clevidence B A, Kurilich A C.Anthocyanin kinetics are dependent on anthocyanin structure[J].British Journal of Nutrition, 2012, 107 (4) :504-509.
[6] Huang Huipei, Shih Y W, Chang Y C, et al.Chemoinhibitory effect of mulberry anthocyanins on melanoma metastasis involved in the Ras/PI3K pathway[J].Journal of Agricultural and Food Chemistry, 2008, 56 (19) :9286-9293.
[7] Shin D Y, LU Jingnan, Kim G Y, et al.Anti-invasive activities of anthocyanins through modulation of tight junctions and suppression of matrix metalloproteinase activities in HCT-116human colon carcinoma cells[J].Oncology Reports, 2011, 25 (2) :567-572.
[8] Wang L S, Sun X D, Cao Y, et al.Antioxidant and pro-oxidant properties of acylated pelargonidin derivatives extracted from red radish[J].Food&Chemical Toxicology, 2010, 48 (10) :2712-2718.
[9] Kausar H, Jeyabalan J, Aqil F, et al.Berry anthocyanidins synergistically suppress growth and invasive potential of human non-small-cell lung cancer cells.[J].Cancer Letters, 2012, 325 (1) :54-62.
[10] Yun J W, Lee W S, Kim M J, et al.Characterization of a profile of the anthocyanins isolated from Vitis coignetiae Pulliat and their antiinvasive activity on HT-29 human colon cancer cells[J].Food and Chemical Toxicology, 2010, 48 (3) :903-909.
[11] Zhang Y.Human tumor cell growth inhibition by nontoxic anthocyanidins, the pigments in fruits and vegetables.[J].Life Sciences, 2005, 76 (76) :1465-1472.
[12] Kamenickova A, Anzenbacherova E, Pavek P, et al.Pelargonidin activates the AhR and induces CYP1A1 in primary human hepatocytes and human cancer cell lines Hep G2 and LS174T[J].Toxicology Letters, 2013, 218 (3) :253-259.
[13] Ferraz M A, Zabaglia L M, Pereira W N, et al.Downregulated Expression of E-cadherin and TP53 in Patients with Gastric Diseases:The Involvement of H.pylori Infection and Its Virulence Markers[J].Journal of Gastrointestinal Cancer, 2016, 47 (1) :20-26.
[14] Rai H C, Ahmed J.A Role of N-Cadherin in Tumor Progression and Prognosis of Epitheliual Malignancy[J].2016, 3 (1) :2393-2915.
[15] 卢昕, 孟庆彬, 邵永胜.CDH17对胃癌细胞侵袭性的影响及其机制[J].中国普通外科杂志, 2015, 24 (10) :1406-1410. [16] 汤光华.胃癌中Slug和E-cadherin表达及其临床意义[J].武汉大学学报, 2015, 36 (4) :536-540. [17] Xia R, Jin F Y, Lu K, et al.SUZ12 promotes gastric cancer cell proliferation and metastasis by regulating KLF2 and E-cadherin[J].Tumor Biology, 2015, 36 (7) :5341-5351.
[18] Jiang S B, He X J, Xia Y J, et al.MicroRNA-145-5p inhibits gastric cancer invasiveness through targeting N-cadherin and ZEB2 to suppress epithelial-mesenchymal transition[J].Oncotargets&Therapy, 2016, 9:2305-2315.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
