Determination of total anthraquinone ( emodin and physcion) in Yizhikang capsule by high performance liquid chromatography
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摘要: 建立益之康胶囊中总蒽醌含量测定的高效液相色谱法。采用HPLC测定益之康胶囊中2种蒽醌类成分大黄素和大黄素甲醚的含量;流动相为甲醇-0.1%磷酸溶液（75∶25,v/v）,柱温30℃,流速1.0 m L/min,检测波长254 nm。大黄素和大黄素甲醚分别在0.0540.65μg和0.0220.27μg范围内呈良好的线性关系,相关系数分别为0.9999和0.9999,检出限分别为0.86μg/g和0.87μg/g,平均回收率分别为97.20%（RSD=2.26%）和99.96%（RSD=2.18%）。结论:该方法简便、准确、可靠。
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关键词:
- 益之康胶囊 /
- 大黄素 /
- 大黄素甲醚 /
- 总蒽醌 /
- 高效液相色谱法(HPLC)
Abstract: Objective: To establish a High Performance Liquid Chromatography ( HPLC) method for determining the totalanthraquinone in Yizhikang capsule.Methods: The contents of emodin and physcion were determined by a HPLC method.The emodin and physcion were separated on a Waters Sunfire C18 column ( 250 mm × 4.6 mm, 5 μm) using a mobile phase made up of methanol and 0.1% phosphoric acid solution ( 75∶ 25, v/v) at a flow rate of 1.0 m L/min and detected by a UV detector at a wavelength of 254 nm.The column temperature was set at 30 ℃.Results: The response showed good linear relationship in the range of 54.09649.05 μg/m L for emodin, 22.11 ~ 265.31 μg/m L for physcion with all the coefficients of correlation ( R2) =0.9999. The detection limits ( LODs) were 0.86 μg/g for emodin, 0.87 μg/g for physcion, respectively.The average recoveries of emodin and physcion were 97.20% ( RSD = 2.26%) and 99.96% ( RSD = 2.18%) , respectively. Conclusions: This method is simple, accurate, reliable and reproducible and can be used for the quality control of yizhikang capsule. -
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