Inhibition of biofilm formation in Pseudomonas fluorescens by cinnamaldhyde
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摘要: 本文拟研究肉桂醛在非抑菌浓度条件下对荧光假单胞菌（Pseudomonas fluorescens）FML05-2生物膜形成的抑制作用。首先测定了肉桂醛对模式细菌紫色杆菌（Chromobacterium violaceum）CVO26紫色素产生的影响;再对荧光假单胞菌FML05-2形成的生物膜以及与生物膜形成有关的重要因素胞外多糖进行了测定。结果表明:在非抑菌浓度40、20μg/m L条件下,肉桂醛对紫色杆菌CVO26紫色素的产生具有抑制作用,抑制率分别为31.53%、17.90%;对荧光假单胞菌FML05-2生物膜形成的抑制率分别为44.22%、21.77%;对荧光假单胞菌FML05-2胞外多糖产生的抑制率分别为15.72%、5.34%。因此,肉桂醛在非抑菌浓度条件下对荧光假单胞菌FML05-2生物膜的形成具有抑制作用。Abstract: To study the inhibition of development of biofilm in Pseudomonas fluorescens FML05-2 by cinnamaldhyde at the non inhibitory concentration. Firstly, it was studied that the effect of the cinnamaldhyde on the purple pigment production by Chromobacterium violaceum CVO26 as model bacteria, then determined the biofilm formation and extracellular polysaccharide ( EPS) production that was important factors correlated with the biofilm formation in P. fluorescens FML05-2. The results indicated that the cinnamaldhyde inhibited the purple pigment production of C. violaceum CVO26, the inhibition rates were31.53% and 17.90%, respectively.The inhibition rates of the biofilm development were 44.22%, 21.77%, the inhibition rates of EPS production were 15.72%, 5.34% in P. fluorescens FML05-2 at the non inhibitory concentration of 40 μg/m L and20 μg/m L. The results suggest that the cinnamaldhyde can inhibit the development of biofilm in P.fluorescens FML05-2 at the non inhibitory concentration
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