Optimization of subcritical water extraction for total phenolics from pomegranate ( Punica granatum L.) leaves by response surface methodology
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摘要: 本研究采用亚临界水萃取法提取石榴叶总酚,并通过响应面法优化其提取工艺参数。在单因素实验的基础上,根据响应面Box-Behnken实验设计原理,采用三因素三水平的分析法,选取提取温度、提取时间、液料比为自变量,考察其对石榴叶提取物中总酚含量的影响,并通过方差分析优化其工艺。结果表明,回归方程对实验拟合较好,可以对石榴叶中总酚含量进行很好的分析和预测;优化后的最佳工艺条件为:提取温度134℃、提取时间31 min、液料比52∶1 m L/g。在该条件下,石榴叶总酚含量为169.24 mg GAE/g,与预测值173.31 mg GAE/g基本一致。通过与溶剂加热回流法相比较,亚临界水提取可明显提高石榴叶总酚的含量,并缩短提取时间。Abstract: The aim of this study was to optimize the technology parameters of subcritical water extraction for extracting total phenolics from pomegranate leaves by response surface methodology. On the basis of single-factor experiments, three-factors and three-level response surface methodology was applied to investigate the effects of extraction temperature, extraction time and solvent/solid ratio on the total phenolics contents. The results showed that model regression was significant, and the established regression equation for phenolics contents had an excellent of fit.The optimized extraction temperature was 134 ℃, extracting time was 31 minutes, and solvent/solid ratio was 52∶ 1 m L/g.Under the optimized conditions, the total phenolics content was 169.24 mg GAE/g, which was consistent with the predictive value of 173.31 mg GAE/g. In addition, the total phenolic content of the optimized extracts was higher than the reflux extraction using aqueous-organic solvent, and the time was shortened.
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[1] 邱涛涛, 王华, 毛世红.石榴叶总酚测定及提取工艺研究[J].食品科学, 2009, 30 (10) :131-134. [2] 杜欣, 叶盛英.石榴叶和石榴皮药理活性研究进展[J].天津药学, 2007, 19 (2) :64-66. [3] Zhang L, Gao Y, Zhang Y, et al.Changes in bioactive compounds and antioxidant activities in pomegranate leaves[J].Scientia Horticulturae, 2010, 123 (4) :543-546.
[4] Wang R, Ding Y, Liu R, et al.Pomegranate:constituents, bioactivities and pharmacokinetics[J].Fruit, Vegetable and Cereal Science and Biotechnology, 2010, 4 (2) :77-87.
[5] 林佳, 李琰, 徐丽珍.石榴叶的化学成分研究[J].中南药学, 2005, 3 (2) :70-72. [6] 刘志国, 赵文亚, 刘洪波, 等.石榴茶提取物对小鼠抗疲劳抗氧化作用的研究[J].食品研究与开发, 2013 (11) :45-47. [7] Elfalleh W, Hannachi H, Tlili N, et al.Total phenolic contents and antioxidant activities of pomegranate peel, seed, leaf and flower[J].Journal of Medicinal Plants Research, 2012, 6 (32) :4724-4730.
[8] Wang C, Shi L, Fan L, et al.Optimization of extraction and enrichment of phenolics from pomegranate (Punica granatum L.) leaves[J].Industrial Crops and Products, 2013, 42:587-594.
[9] 张立华, 张元湖, 于军伟, 等.超声波辅助提取石榴叶单宁的工艺[J].农业工程学报, 2009 (s1) :164-167. [10] Herrero M, Cifuentes A, Iba1ez E.Sub-and supercritical fluid extraction of functional ingredients from different natural sources:Plants, food-by-products, algae and microalgae:A review[J].Food Chemistry, 2006, 98 (1) :136-148.
[11] 郑光耀, 薄采颖, 张景利.亚临界水萃取技术在植物提取物领域的应用研究进展[J].林产化学与工业, 2010, 30 (5) :108-114. [12] He L, Zhang X, Xu H, et al.Subcritical water extraction of phenolic compounds from pomegranate (Punica granatum L.) seed residues and investigation into their antioxidant activities with HPLC-ABTS+assay[J].Food and bioproducts processing, 2012, 90 (2) :215-223.
[13] 薄采颖, 郑光耀, 陈琰, 等.松树皮多酚的亚临界水提取及抗氧化活性初探[J].林产化学与工业, 2011, 31 (6) :73-77. [14] Xu H, Wang W, Jiang J, et al.Subcritical water extraction and antioxidant activity evaluation with on-line HPLC-ABTS+·assay of phenolic compounds from marigold (Tagetes erecta L.) flower residues[J].Journal of food science and technology, 2015, 52 (6) :3803-3811.
[15] Shaddel R, Maskooki A, Haddad-Khodaparast M H, et al.Optimization of extraction process of bioactive compounds from Bene hull using subcritical water.Food Science and Biotechnology, 2014, 23 (5) :1459-1468.
[16] 王彩云.石榴叶多酚的提取富集工艺及其药理作用研究[D].北京:北京林业大学, 2013. [17] Bala I, Bhardwaj V, Hariharan S, et al.Analytical methods for assay of ellagic acid and its solubility studies[J].Journal of Pharmaceutical&Biomedical Analysis, 2006, 40 (1) :206-210.
[18] 汪泽, 崔丽虹, 唐冰, 等.响应面法优化菠萝叶纤维多酚提取工艺[J].食品工业科技, 2017, 38 (1) :219-223. [19] 陈书勤, 黄健泉, 黄康宁, 等.响应曲面法在优化果糖和葡萄糖色谱分离中的应用[J].轻工科技, 2011 (10) :20-21. [20] Yang L, Yin P, Fan H, et al.Response surface methodology optimization of ultrasonic-assisted extraction of Acer Truncatum leaves for maximal phenolic yield and antioxidant activity[J].Molecules, 2017, 22 (2) :232.
[21] 陈婷, 徐文斌, 洪怡蓝, 等.响应面法优化甜叶菊残渣中总黄酮提取工艺及抗氧化活性[J].食品科学, 2016, 37 (18) :52-57.
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