Isolation, identification and enzymology properties of an agarase-producing strain
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摘要: 本研究通过初筛、复筛从青岛近海沉积物中分离出一株具有琼胶酶活性的菌株,该菌的最佳发酵时间为26 h,对该菌培养基进行卢戈氏碘液染色后菌落周围出现明显透明圈,镜检观察菌株的革兰氏染色结果表明,该菌为革兰氏阴性菌。经过鉴定,该菌株为不动杆菌（Acinetobacter sp.）,命名为Acinetobacter sp.LXK,对该菌的粗酶学特性进行初步研究,结果显示,该酶的最适反应温度为40℃,最适底物浓度为0.5%,最适反应体系p H为8.0,K+和Ca2+对酶解反应有促进作用,Fe2+、Mn2+和Ba2+对酶解反应有不同程度的抑制作用。Abstract: An agar-decomposing strain was isolated from Qingdao offshore deposits. The optimal fermentation time was 26 h, transparent zones appeared after staining with lugol 's iodine. The bacteria was gram-negative and classified in the genus Acinetobacter, named as Acinetobacter sp.LXK.By studying the primary properties of agarase and study showed that: the optimal p H and temperature for enzymolysis were p H8.0 and 40 ℃ respectively, the optimum concentration of substrate was 0.5%, the enzyme was activate by K+, Ca2 +and inhabited by Fe (2+）, Mn（2+）, Ba（2+）.
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Keywords:
- agarase /
- marine bacteria /
- screening /
- identification
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