Optimization of the cultivation conditions for bacteriocin production of Lactococcus raffinolactis Y-12
-
摘要: 目的:以分离自独山盐酸菜产细菌素棉籽糖乳球菌Y-12为研究对象,对其产细菌素培养条件进行优化。方法:利用单因素和Plackett-Burman实验确定发酵温度、发酵时间、初始p H和葡萄糖浓度等发酵条件水平值,在单因素和Plackett-Burman实验的基础上,采用Box-Behnken法设计三因素三水平实验进行响应面优化,确定菌株Y-12产细菌素最佳发酵条件。结果:菌株Y-12产细菌素最佳发酵条件为:发酵温度35℃、发酵时间36 h、接种量2%、蛋白胨10.62 g/L、葡萄糖31.61 g/L,初始p H6.46,其他成分（同MRS培养基）保持不变。在此条件下,细菌素抑菌圈直径达16.87 mm,比优化前（14.12 mm）提高19.5%。结论:在最优条件下获得实验结果与模型预测值吻合,说明所建立回归方程模型切实可行。
-
关键词:
- 棉籽糖乳球菌 /
- 细菌素 /
- 响应面 /
- Plackett-Burman设计 /
- 优化
Abstract: Objective: Lactococcus raffinolactis Y-12 was isolated from Dushan pickled Chinese cabbage, and the fermentation conditions of bacteriocin produced from it were optimized.Methods: The factors including fermentation time, temperature, initial p H, glucose and so on, that affected the yield of bacteriocin, were studied in single factor and Plackett-Burman optimization test.On the basis of single factor test and Plackett-Burman treatment, the conditions for bacteriocin production were explored by Box-Behnken design involving the peptone, glucose and initial p H at three levels in combination with response surface methodology. Results: The optimal combination of the medium constituents for bacteriocin production were as follows:fermentation temperature 35 ℃, fermentation time 36 h, inoculums concentration 2%, 10.62 g/L peptone, 31.61 g/L glucose, initial p H6.46, the other composition of MRS medium were unchange.Under the optimal condition, the inhibitory zone diameter was 16.87 mm, than before optimization ( 14.12 mm) to improve 19.5%.Conclusion: The experimental results were in agreement with the prediction values, indicated the established regression model was feasible. -
[1] 侯亚文, 易华西, 杨艳艳, 等.产细菌素乳酸菌筛选方法的研究进展[J].食品与发酵工业, 2013, 39 (3) :129-133. [2] Deegan L H, Cotter P D, Hill C, et al.Bacteriocins:Biological tools for bio-preservation and shelf-life extension[J].International Dairy Journal, 2006, 16 (9) :1058-1071.
[3] 饶瑜, 常伟, 唐洁, 等.产细菌素乳酸菌在蔬菜发酵制品生物保鲜中的应用[J].食品工业科技, 2013, 34 (16) :392-395, 400. [4] Cotter P D, Hill C, Ross R P.Bacteriocins:developing innate immunity for food[J].Nature Reviews Microbiology, 2005, 3 (10) :777-788.
[5] Reis J A, Paula A T, Casarotti S N, et al.Lactic acid bacteria antimicrobial compounds:characteristics and applications[J].Food Engineering Reviews, 2012, 4 (2) :124-140.
[6] 卢晓黎, 尼海峰.发酵蔬菜功能菌研究与应用进展[J].中国食品学报, 2012, 12 (2) :1-6. [7] Altuntas E G, Cosansu S, Ayhan K.Some growth parameters and antimicrobial activity of a bacteriocin-producing strain Pediococcus acidilactici 13[J].International Journal of Food Microbiology, 2010, 141 (1) :28-31.
[8] 胡美忠, 党丽娟, 陆兆新.Lactobacillus plantarum 163产细菌素食品级培养基筛选及发酵条件优化[J].食品科学, 2016 (15) :165-170. [9] 王娜娜, 李婉, 杜金城, 等.乳酸乳球菌KLDS4.0325产细菌素最佳培养条件的研究[J].食品工业科技, 2016, 37 (14) :187-190. [10] 徐顾榕, 陈志伟, 钟小廷, 等.响应面法优化蜡样芽孢杆菌产类细菌素培养基[J].食品工业科技, 2016 (19) :212-217. [11] 佟世生, 解洛香, 徐乐, 等.植物乳杆菌代谢产细菌素的培养基优化[J].现代食品科技, 2012, 28 (2) :152-155. [12] 王娜娜, 李婉, 于上富, 等.L.lactis KLDS4.0325产细菌素发酵培养基的响应面优化[J].食品工业科技, 2016, 37 (7) :137-142. [13] 于微, 高学军, 马春丽, 等.干酪乳杆菌产细菌素的生物学特性分析[J].安徽农业科学, 2014, 42 (32) :11542-11543. [14] 胡彦新, 刘小莉, 王英, 等.香肠乳杆菌 (Lactobacillus farcimini) FM-MM4产细菌素发酵条件和培养基优化[J].食品工业科技, 2016, 37 (10) :255-259. [15] Zhu X, Zhao Y, Sun Y, et al.Purification and characterisation of plantaricin ZJ008, a novel bacteriocin against Staphylococcus, spp.from Lactobacillus plantarum, ZJ008[J].Food Chemistry, 2014, 165 (3) :216-223.
[16] 申乃坤, 王青艳, 秦艳, 等.木薯粉同步糖化发酵 (SSF) 产丁二酸[J].微生物学通报, 2014, 41 (8) :1507-1515. [17] 李亚玲.乳酸片球菌细菌素的分离纯化及理化性质研究[D].天津:天津大学, 2007. [18] 滕志利.产广谱细菌素乳酸菌的筛选及其细菌素特性、发酵条件的研究[D].大连:大连工业大学, 2013. [19] 满丽莉, 孟祥晨, 王辉, 等.群体感应系统在乳酸菌产细菌素中的作用[J].食品科学, 2011, 32 (13) :360-364. [20] 刘国荣, 张郡莹, 王成涛, 等.响应面法优化弯曲乳杆菌RX-6代谢产细菌素的发酵培养基组成[J].食品科技, 2013 (3) :2-8. [21] Liu J Z, Weng L P, Zhang Q L, et al.Optimization of glucose oxidase production by Aspergillus niger in a benchtop bioreactor using response surface methodology[J].World Journal of Microbiology and Biotechnology, 2003, 19 (3) :317-323.
[22] Sharma S, Malik A, Satya S.Application of response surface methodology (RSM) for optimization of nutrient supplementation for Cr (VI) removal by Aspergillus lentulus AML05[J].Journal of Hazardous Materials, 2009, 164 (2) :1198-1204.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
