Preparation and characterization of phytic acid molecularly imprinted polymer and its adsorption performance
-
摘要: 以植酸为模板分子,甲基丙烯酸（MAA）为功能单体,N,N’-乙烯基丙烯酰胺（EBA）为交联剂,过硫酸铵为引发剂,采用本体聚合法制备对植酸具有高特异选择性的植酸分子印迹聚合物（MIPs）。通过扫描电镜观察了分子印迹聚合物表面的结构,利用红外光谱进一步对其化学结构进行表征。通过动态吸附、静态吸附和选择性吸附考察了其吸附性能。研究表明植酸分子印迹聚合物对植酸的吸附高于对肌醇的吸附,植酸在印迹聚合物中的印迹因子为1.70,而与植酸结构相似物肌醇的印迹因子为1.46。解吸80 min,MIPs解吸达到稳定,解吸率达到89.59%。植酸分子印迹聚合物对植酸的吸附特异性强,对植酸的吸附量为21.38μmol·g-1,解吸效果好,进行吸附-解吸附循环5次后,植酸印迹聚合物的性能稳定,对植酸吸附容量为65.62μmol·g-1,是第一次吸附容量的90.00%,可重复使用。Abstract: The molecularly imprinted polymer ( MIPs) were prepared by bulk polymerization using phytic acid ( PA) as template molecule, MAA as functional monomer, N, N'-Ethylenebis ( acrylamide) as crosslinker, Ammonium persulphate as initiator.Surface structure of molecularly imprinted polymers was observed by SEM, the chemical structure of MIPs was characterized by FT-IR. MIPs adsorption performance were investigated by dynamic adsorption, static adsorption and selective adsorption experiments. The experimental results showed that the PA MIPs exhibited good adsorption and recognition abilities, with imprinted factors of 1.70, while myo-inositol ( MI) was 1.46.The adsorption reaction reached equilibrium after 80 min, and the desorption ratio of ractopamine reached 89.59%.The experimental results showed that MIPs not only have specific recognition selectivity, but also have good desorption property for PA, and the adsorption capacity of phytic acid was 21.38 μmol·g-1.After adsorption-desorption 5 times, MIPs were stable and reusable, the adsorption capacity of phytic acid was 65.62 μmol·g-1, which was 90.00% of the first adsorption capacity and could be cycle used.
-
Keywords:
- phytic acid /
- molecularly imprinted polymer /
- characterization /
- adsorption performance /
- desorption
-
[1] 元晓梅, 蒋明蔚, 胡正芝.粮谷食品中植酸检测方法的研究[J].食品与发酵工业, 1997, 23 (1) :25-31 [2] 吴澎, 田纪春, 王凤成.谷物中植酸及其应用的研究进展[J].中国粮油学报, 2009, 24 (3) :137-143. [3] Zhou JR, Erdman JW.Phytic acid in health and disease[J].Critical Reviews in Food Science and Nutrition, 1995, 35 (6) :495-508.
[4] 吴汉民.新保鲜剂植酸一低浓度限量应用的卫生安全性考证[J].食品与发酵工业, 1991, 6 (4) :26-32. [5] Midorikawa K, Murata M, Oikawa S, et al.Protective effect of phytic acid on oxidative DNA damage with reference to cancer chemoprevention[J].Biochem Biophysical Research Communications, 2001, 288 (3) :552-557.
[6] 王天丰, 曹茜茜, 撒悦, 等.植酸作为螯合剂去除根管玷污层的效力及其对牙本质化学组成的影响[J].口腔医学研究杂志, 2016, 32 (4) :374-378. [7] Katherine L, Em Pson TP, Labuza.Phytic Acid as a Food Antioxidant[J].Journal of Food Science, 1991, 56 (2) :560-563.
[8] Raboy V.Seeds for a better future:Low phytate’grains help to overcome malnutrition and reduce pollution[J].Trends Plant Science, 2001, 6 (10) :458-462.
[9] Guttieri MJ, Peterson KM, Souza EJ.Agronomic performance of low phytic acid wheat[J].Crop Science, 2006, 46 (6) :487-493.
[10] Ravindran V.Phytate occurance, bio-availability and implications in poultry nutrition[J].Poutry Avian Biology Reviewws, 1995b, 6 (2) :125-134.
[11] Viveros A, Centeno C, Brenes A, et al.Phytase and acid phosphatase activities in plant feedstuffs[J].Journal of Agricultural&Food Chemistry, 2000, 48 (9) :4009-4013.
[12] 张瑞.植酸的提取、分离与纯化工艺研究[D].安徽:合肥工业大学, 2013. [13] Vlatakis G, Andersson L I, Muller R, et al.Drug assay using antibody mimics made by molecular imprinting[J].Nature, 1993, 361 (3) :645-647.
[14] 李蓉, 储大可, 高永清, 等.复合分子印迹固相萃取-HPLC-MS/MS法测定植物源性食品中多种农药残留[J].分析实验室, 2015 (8) :907-912. [15] 吴洪, 赵艳艳, 喻应霞, 等.分子印迹壳聚糖膜分离手性苯丙氨酸[J].功能高分子学报, 2007, 19 (3) :262-266. [16] 李国良, 姚伟, 韩建光, 等.农药丙溴磷分子印迹聚合物微球的制备与结合性能研究[J].分析测试学报, 2009, 28 (2) :207-211. [17] 姚婷, 李腾飞, 秦玉昌, 等.分子印迹表面等离子共振传感器在食品安全检测中的最新研究进展[J].分析测试学报, 2015, 34 (2) :37-244. [18] 唐志民, 马新宾.林可霉素分子印迹材料的制备及吸附性能[J].中国粉体技术, 2016 (5) :63-67. [19] Giuseppe C, Manuela C, Ortensia IP.Gastro-intestinal sustained release of phytic acid by molecularly imprinted microparticles[J].Pharmaceutical Development and Technology, 2010, 15 (5) :526-531.
[20] 贺湘, 高清涌, 刘淑卿.红外微量溴化钾压片法及其应用[J].化学世界, 1980 (5) :17-19. [21] 任鸿均.植酸、植酸钙和肌醇[J].化工科技市场, 2003, 26 (4) :25-30. [22] Giuseppe C, Manuela C, Ortensia IP.Gastro-intestinal sustained release of phytic acid by molecularly imprinted microparticles[J].Pharmaceutical Development and Technology, 2010, 15 (5) :526-531.
[23] 赵娜, 胡小玲, 管萍, 等.乳液聚合法制备红霉素分子印迹聚合物微球及其吸附性能[J].物理化学学报, 2014, 30 (1) :121-128. [24] 杨挺, 吴银良, 李存, 等.莱克多巴胺表面分子印迹材料的制备、表征及吸附性能[J].中国农业科学, 2013, 46 (6) :1256-1262. [25] 雷福厚, 赵慷, 李小燕, 等.脱氢枞胺分子印迹聚合物的吸附性能研究[J].精细化工, 2010, 27 (1) :11-15.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
