Determination of solubility and metastable zone of Rebaudioside A in ethanol-isopropanol-water system
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摘要: 采用动态稳定法测定了莱鲍迪甙A（RA）在乙醇-异丙醇溶液中的溶解度和超溶解度曲线,探讨了溶剂组分变化等因素对莱鲍迪甙A在乙醇-异丙醇-水溶液中的溶解度影响,并利用多项式经验方程对溶解度及超溶解度与温度进行了关联。结果表明在060℃范围内莱鲍迪甙A在乙醇-异丙醇-水溶液中的溶解度随温度的升高而增加,并随着异丙醇用量的增加而减小,且随着水用量比例的增加而增加;介稳区宽度在47℃,介稳区宽度则随温度的升高而减小;多项式经验方程关联误差小,为乙醇-异丙醇-水体系重结晶分离莱鲍迪苷A提供了重要的基础数据。Abstract: The solubility and supersolubility curves of Rebaudioside A ( RA) in ethanol-isopropanol-water system were determined using the dynamic stabilization method. The solubility of RA at the different composition of solvent was also measured.Furthermore, the polynomial empirical equation was used to correlate the experimental data. The results showed that the solubility of RA increased with the temperature in ethanol-isopropanol-water system and decreased with the increasing of isopropanol's proportion, furthermore, it increased with increasing of H2O's proportion. Moreover, the metastable zone width decreased with the increasing of temperature from 0 to 60 ℃.The metastable zone width was about 4 to 7 ℃. The error of polynomial empirical equation was small and it provided essential data for the crystallization process of Rebaudioside A.
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