Hemostatic activity of cod skin gelatin-Ca2+ complex hemostatic powder
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摘要: 利用超滤技术制备三个不同分子量段（Mr>50 ku、10 ku<Mr<50 ku、Mr<10 ku）的鳕鱼皮明胶,分别与Ca2+复配为明胶-Ca2+复配止血粉。通过液体吸收倍率、大鼠体内、外止血实验,探究了不同分子量鳕鱼皮明胶-Ca2+复配止血粉的止血性能,并对其止血机制做初步探讨。结果表明:高分子量鳕鱼皮明胶-Ca2+复配止血粉的液体吸收倍率最大,且能显著（p<0.05）缩短体外凝血时间（24 s）,降低大鼠股动脉出血时间（114 s）和肝创面出血时间（97 s）。高分子量鳕鱼皮明胶-Ca2+复配止血粉能显著（p<0.01）缩短活化部分凝血活酶时间（activated partial thromboplastin time,APTT）,主要影响内源性凝血途径;并能促进血小板聚集,通过促进大鼠血小板第四因子（Rat Platelet Factor 4,PF4）、P-选择素（Rat P-Selection）和血栓素B2（Rat Thromboxane B2,TXB2）的释放来缩短凝血时间。高分子量明胶-Ca2+复配止血粉可以作为一种有效的止血材料投入到止血产品的工业化生产中。Abstract: Three cod skin-derived gelatin with different molecular weights ( Mr > 50 ku, 10 ku < Mr < 50 ku, Mr < 10 ku) were obtained by ultrafiltration technology.They were chelated with calcium to obtain a gelatin-Ca2+hemostatic powder material.The hemostatic properties of different molecular weight gelatin-Ca2+compound hemostatic powder were studied by fluid absorption ratio and hemostatic experiments in vivo and in vitro, and the hemostatic mechanism was also discussed.The results showed that the liquid absorption capacity of high molecular weight group was the highest, high molecular weight gelatin-Ca2+hemostatic powder can significantly ( p < 0.05) shorten the coagulation time in vitro ( 24 s) , reduce rat femoral artery bleeding time ( 114 s) and liver wound bleeding time ( 97 s) . High molecular weight group significantly ( p < 0.01) reduced APTT levels, and affected the endogenous coagulation pathways. High molecular weight gelatin-Ca2+hemostatic powder also promoted platelet aggregation, and increase the release of platelet active factors, such as platelet factor 4 ( PF4) , P-selectin, and thromboxane B2 ( TXB2) .Thus, high molecular weight gelatin-Ca2+compound hemostatic powder could be considered an effective absorbable hemostatic material in the industrialized production.
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Keywords:
- cod skin gelatin /
- Ca2+ /
- compound hemostatic powder /
- hemostatic properties
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