Development of quantitative detection techniques of genetically modified organisms
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摘要: 转基因作物的种类以及含有转基因成分的产品在日益增长,转基因产品的安全性也越来越被社会公众广泛关注。世界各国均颁布转基因安全管理、标识法规,对转基因生物及其加工产品进行标识和管理。如何准确、稳定地对转基因产品中的转基因成分进行定量,已成为转基因产品检测技术研究的重要方向。本文综述了竞争性PCR、荧光定量PCR以及数字PCR等定量检测技术在转基因成分检测中应用及研究进展,讨论了目前各类转基因定量检测技术的优缺点,并在此基础上展望了定量检测技术在转基因成分检测领域的发展前景。Abstract: The concern of public has been more and more focus on the safety of the Genetically Modified Organisms ( GMO) as the rapid development of transgenic techniques and uncertain effect to human beings and environment. Different countries and organizations have declared kinds of safety and labeling laws to achieve the proper regulation to the commercialization of the GM products.In this manner, the achievement of stable and accurate quantitation of GMO content within different kinds of commercialized product has become the key point of the development of GMO detection techniques. In this paper, the application and development of different quantitative detection methods were summarized, such as competitive PCR, real-time PCR, digital PCR and etc. Meanwhile, the advantages and drawbacks of different detection methods were discussed and the future of the quantitative detection methods in the area of GMO content quantitation were predicted.
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Keywords:
- transgenic /
- quantitative detection /
- PCR /
- digital PCR
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