Survey of the pollution of Vibrio parahaemolyticus from three shellfish products in Zhangzhou City
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摘要: 调查漳州市售主要贝类中副溶血性弧菌的带菌状况,为消费者提供食品安全风险参考。从漳州市区的8大市场无菌采集花蛤、文蛤和蛏的新鲜样品;用硫代硫酸盐柠檬酸盐胆盐蔗糖琼脂培养基(Thiosulfate citrate bile salts sucrose agar culture medium,TCBS)分离纯化疑似菌落,聚合酶链式反应(Polymerase Chain Reaction,PCR)扩增不耐热溶血素基因(thermolabile hemolysin,tlh)的特异片段;并对分子检测阳性的菌株进行生化鉴定。结果表明,采集到53份花蛤、50份文蛤和42份蛏的样品,经过TCBS筛选分别得到37株、45株和36株疑似菌落,分离率分别为69.8%、90.0%和85.7%;PCR确定副溶血性弧菌阳性率分别为35.8%(19/53)、30.0%(15/50)和54.8%(23/42);革兰氏染色和主要生化实验确定疑似菌均为副溶血性弧菌,符合率达100%。漳州市所售水产品受副溶血性弧菌污染较为严重,市民食用需严格无害化处理,避免不同种类水产品间的交叉污染。Abstract: To analyze the carrying status of Vibrio parahaemolyticus of shellfish products in Zhangzhou City and provide reference for the food safety problem of shellfish. The fresh sample were collected by sterile operation from 8 markets in Zhangzhou City.The suspected colony was obtained by culturing on TCBS plates and detected using PCR amplification of a specific tlh gene. The positive strain was verified using biochemical test and Gram staining. 53 Venerupis philippinarum, 50 Meretrix and 42 Scalprum samples were collected from 8 main markets, 37 Venerupis philippinarum, 45 Meretrix and 36 Scalprum suspected colony were screening with TCBS selective medium.The separation rate were 69.8%, 90.0% and 85.7% for Venerupis philippinarum, Meretrix and Scalprum, respectively. PCR positive rate were 35.8% ( 19/53) , 30.0% ( 15/50) and54.8% ( 23/42) . These positive strain were confirmed by Gram staining and biochemical test. The shellfish products in Zhangzhou City are polluted by Vibrio parahaemolyticus, seafood should be strictly disposed, to avoid cross contamination between different types of aquatic products.
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Keywords:
- Vibrio parahaemolyticus /
- Venerupis philippinarum /
- Meretrix /
- Scalprum /
- Zhangzhou City
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