Study on polyphenol purification by macroporous resin and inhibition activity to pancreatic amylase of polyphenol from Docyniaindica (Wall.) Dcne leaves
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摘要: 研究栘叶多酚的大孔吸附树脂纯化工艺,并考察其纯化前后对胰淀粉酶的抑制作用。通过静态吸附解析实验筛选出吸附性能和解吸性能良好的D-101型大孔吸附树脂,进一步研究其纯化多酚的工艺条件和技术参数。结果显示:上样溶液质量浓度为25 mg/m L,上样流速为1 m L/min,以10 BV进行上样,以50%的乙醇溶液为解析剂,洗脱流速为3 m L/min,洗脱体积为8 BV进行洗脱,栘叶浸膏中多酚的含量由原来的39.7%提高到91.89%。纯化后,栘叶多酚对胰淀粉酶的抑制率高于纯化前,且纯化前后对胰淀粉酶的抑制率均高于100 mg/m L大黄提取物对胰淀粉酶的抑制效果,说明栘叶多酚对胰淀粉酶有明显的抑制效果,且纯化有利于提高栘叶多酚对胰淀粉酶的抑制作用。Abstract: To propose an optimal purification process for polyphenol extracted from Docyniaindica( Wall.) Dcne leaves and investigate the inhibition of pancreatic amylase before and after purification.Macroporous resins D-101 was found more suitable to purify polyphenols by comparing their static adsorption and desorption capacities with those of two other kinds of macroporous resin. The purification process of D-101 was determined after static and dynamic experiment.When the D-101 was adsorbed with the maximum sample volume of 10 BV sample solution containing 25 mg / m L extractive at a rate of 1 m L / min,and desorpted with 8 BV of 50% ethanol at a flow rate of3 m L / min,the content of the total polyphenol could be increased from 39.7% to 91.89%.The method proved to be simple and feasible for purification of total polyphenols extracted from Docyniaindica( Wall.) Dcne leaves. After purification,the inhibitory rate of total polyphenols extracted from Docyniaindica( Wall.) Dcne leaves increased,and the inhibition rate before and after purification was higher than that of 100 mg / m L rhubarb extract on pancreatic amylase,indicating that polyphenols of Docyniaindica( Wall.) Dcne leaves had obvious inhibiting effect on pancreatic amylase,which the purification can improve.
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Keywords:
- Docyniaindica(Wall; /
- ) Dcne; /
- polyphenol; /
- macroporous resin; /
- purification; /
- pancreatic amylase;
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[1] 中国科学院《中国植物志》编委会.中国植物志[M].第36卷.北京:科学出版社,1974:346-349. [2] 罗强,刘建林,蔡光泽.中国栘属(Docynia Dcne.)一新种-长爪栘[J].植物研究,2011,31(4):389-391. [3] Huang Ji,Pei Shengji,Long Chunlin.An ethnobotanical study of medicinal plants used by the Lisu people in Nujiang,Northwest Yunnan,China[J].Economic Botany,2004,58(1):S253-S264.
[4] 官智,潭颂德,苏镜娱,等.云南栘黄酮成分研究[J].天然产物研究与开发,2000,12(3):34-37. [5] Nguyen T T L,Hoang T M T,Vu T H T,et al.Anti-obesity and body weight reducing effect of Docyniaindica(Wall.)Decne fruit extract fractions in experimentally obese mice[J].VNU Journal of Science,Natural Sciences and Technology,2011,27(2):125-133.
[6] Hazarika T K,Lalramchuana,Nautiyal B P.Studies on wild edible fruits of Mizoram,India used as ethno-medicine[J].Genetic Resources and Crop Evolution,2012,59(8):1767-1776.
[7] 吴京.傣药栘叶的HPLC指纹图谱研究及其抗氧化分析[D].成都:四川师范大学生命科学学院,2014:56-57. [8] 刘海霞,刘刚,张晓喻,等.栘属植物多酚的含量测定与比较[J].食品科学,2014,35(24):295-300. [9] Sushma K,Shantibaladevi G A.Determination of antioxidant activity and vitamin C of some wild fruits of Manipur[J].Bioscan,2010,5(3):501-504.
[10] Nguyê~n Thi·Minh Thu’.Study on antibacterial activity toward antibiotic resistant bacteria(Moraxella catarrhalis)causing upper respiratory in human of Docynia fruit extract(Docyniaindica)[D].Vietnam:Vietnam National University,2012.
[11] 李健,张令文,刘宁,等.大孔吸附树脂法纯化苦瓜皂苷工艺的研究[J].食品工业技术,2007,28(7):127-130. [12] 王雅,樊明涛,赵萍,等.大孔树脂对沙枣多酚的动态吸附解析性能研究[J].西北农林科技大学学报,2010,38(12):215-220. [13] 冯进,李敏,曾晓雄,等.大孔树脂纯化蓝莓叶多酚及其组成分析[J].食品科学,2013,34(10):86-91. [14] 贺金娜,曹栋,史苏佳.大孔树脂纯化苹果多酚的工艺优化[J].食品与发酵工业,2014,40(5):135-141. [15] 张智,于震,王振宇,等.落叶松树皮多酚纯化工艺研究[J].食品工业科技,2014,35(5):187-191. [16] 刘杰超,焦中高,王思新.苹果多酚提取物对α-淀粉酶和α-葡萄糖苷酶的抑制作用[J].果树学报,2011,28(4):553-557. [17] Krentz A J,Bailey C J.Oral antidiabetic agents:Current rolein type 2 diabetes mellitus[J].Drugs,2005,65:385-411.
[18] 李波,包怡红,高锋,等.红松松球鳞片多酚对α-淀粉酶和α-葡萄糖苷酶的抑制作用[J].食品工业科技,2015,36(1):63-69. [19] Hilda Nyambe-Silavwe,Jose A Villa-Rodriguez,Idolo Ifie,et al.Inhibition of humanα-amylase by dietary Polyphenols[J].Journal of Functional Foods,2015,19:723-732.
[20] 刘京鑫,何云华.不同型号大孔树脂对苹果多酚物质的吸附特性[J].应用化工,2014,43(7):1208-1213. [21] 王旭苹,杨磊,杨小兰,等.大孔树脂纯化酒花多酚及其组成分析[J].食品科学,2013,34(22):15-20. [22] Amar Djeridane,Aicha Hamdi,Wafa Bensania,et al.The in vitro evaluation of antioxidative activity,a-glucosidase and a-amylase enzyme inhibitory of natural phenolic extracts[J].Diabetes&Metabolic Syndrome:Clinical Research&Reviews,2015(9):324-331.
[23] 中华全国供销合作社杭州茶叶研究院.GB/T8313-2008,茶叶中茶多酚和儿茶素类含量的检测[S].北京:中华全国供销合作社,2008. [24] 金莹,孙爱东.大孔树脂纯化苹果多酚的研究[J].食品科学,2007,28(4):160-163. [25] 艾志录,王育红,王海,等.大孔树脂对苹果渣中多酚物质的吸附研究[J].农业工程学报,2007,23(8):245-246. [26] 孙京沙,张朝辉.大叶藻总黄酮的大孔树脂纯化工艺[J].食品科学,2014,35(14):5-10. [27] 袁德彬.复方黑骨藤精制工艺研究[D].四川:四川师范大学生命科学学院,2012. [28] 张俊生,朱士龙,李俊,等.大孔树脂对节节草总黄酮吸附分离特性研究[J].食品工业科技,2012,33(13):236-239. [29] 孙立军.苹果幼果总酚、绿原酸及根皮苷的连续分离及对淀粉酶的抑制[D].西安:陕西师范大学,2013.
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