Application of DNA barcode technology in meat and meat products
-
摘要: DNA条形码技术作为一种新的分子生物学检测技术在鉴定和区分各物种和物种间亲缘关系方面得到了广泛应用,该技术能实现对肉的快速、准确检测。DNA条形码已成为生物学领域发展最迅速的一种技术,该技术基于广泛的物种基因数据库信息,在肉品研究中具有良好的应用前景。本文简述DNA条形码技术的基本原理,并基于DNA水平上与其他相关技术进行比较,综述其在物种鉴定、肉品质量安全、商业欺诈等方面的应用,并对该技术在今后肉品科学研究中的应用进行展望。Abstract: DNA barcoding as a novel molecular biologic technology has been widely used in the identification of distinguishing genetic relationship among species.This technology can be fast and accurate to detect the quality of meat products. DNA barcode has become one of the frontier areas of the most rapidly developing biologic technology,and it is based on a wide range of species gene database information,so it has good prospects in the meat science study.The basic principles of DNA barcode technology was discussed in this review and compared with other technologies based on DNA level.The DNA barcode technology's application in species identification,meat quality,safety analysis and commercial fraud were involved in this paper. The application of DNA barcode technology also was prospected in the future meat science.
-
Keywords:
- DNA barcode /
- meat science /
- species identification /
- quality and safety
-
[1] Hebert P D N,Ratnasingham S,de Waard J R.Barcoding animal life:cytochrome c oxidase subunit 1 divergences among closely related species[J].Proceedings of the Royal Society of London B:Biological Sciences,2003,270(Suppl 1):S96-S99.
[2] 莫帮辉,屈莉,韩松,等.DNA条形码识别ⅠDNA条形码研究进展及应用前景[J].四川动物,2008,27(2):303-306. [3] Hellberg R S R,Morrissey M T.Advances in DNA-based techniques for the detection of seafood species substitution on the commercial market[J].Journal of the Association for Laboratory Automation,2011,16(4):308-321.
[4] Di Pinto A,Marchetti P,Mottola A,et al.Species identification in fish fillet products using DNA barcoding[J].Fisheries Research,2015,170:9-13.
[5] Rona R J,Keil T,Summers C,et al.The prevalence of food allergy:a meta-analysis[J].Journal of Allergy and Clinical Immunology,2007,120(3):638-646.
[6] Sharp M F,Lopata A L.Fish allergy:in review[J].Clinical Reviews in Allergy&Immunology,2014,46(3):258-271.
[7] Filonzi L,Chiesa S,Vaghi M,et al.Molecular barcoding reveals mislabelling of commercial fish products in Italy[J].Food Research International,2010,43(5):1383-1388.
[8] Changizi R,Farahmand H,Soltani M,et al.Species identification reveals mislabeling of important fish products in Iran by DNA barcoding[J].Iranian Journal of Fisheries Sciences,2013,12(4):758-768.
[9] Wong E H K,Hanner R H.DNA barcoding detects market substitution in North American seafood[J].Food Research International,2008,41(8):828-837.
[10] Barbuto M,Galimberti A,Ferri E,et al.DNA barcoding reveals fraudulent substitutions in shark seafood products:the Italian case of“palombo”(Mustelus spp.)[J].Food Research International,2010,43(1):376-381.
[11] Ward R D,Hanner R,Hebert P D N.The campaign to DNAbarcode all fishes,FISH-BOL[J].Journal of Fish Biology,2009,74(2):329-356.
[12] 李新光,王璐,赵峰,等.DNA条形码技术在鱼肉及其制品鉴别中的应用[J].食品科学,2013,34(18):337-342. [13] Ward R D,Costa F O,Holmes B H,et al.DNA barcoding of shared fish s pecies from the North Atlantic and Australasia:minimal divergence for most taxa,but Zeus faber and Lepid opus caudate seach probably constitute two species[J].Aquat Biol,2008,3:71-78.
[14] 王敏,刘荭,黄海,等.DNA条形码技术在深圳鱼肉制品鉴定中的应用[J].食品科学,2015,36(20):247-251. [15] Fajardo V,González I,Rojas M,et al.A review of current PCR-based methodologies for the authentication of meats from game animal species[J].Trends in Food Science&Technology,2010,21(8):408-421.
[16] Verkaar E L C,Nijman I J,Boutaga K,et al.Differentiation of cattle species in beef by PCR-RFLP of mitochondrial and satellite DNA[J].Meat Science,2002,60(4):365-369.
[17] Quinto C A,Tinoco R,Hellberg R S.DNA barcoding reveals mislabeling of game meat species on the US commercial market[J].Food Control,2016,59:386-392.
[18] US Department of Interior(USDI).Endangered and threatened wildlife and plants;endangered status for Gunnison Sage-Grouse.US Department of the Interior,Fish and Wildlife Service[J].Federal Register,2013,78:2485-2538.
[19] Hsieh Y H P,Woodward B B,Ho S H.Detection of species substitution in raw and cooked meats using immunoassays[J].Journal of Food Protection,1995,58(5):555-559.
[20] Ayaz Y,Ayaz N D,Erol I.Detection of species in meat and meat products using Enzyme-Linked Immunosorbent Assay[J].Journal of Muscle Foods,2006,17(2):214-220.
[21] Cawthorn D M,Steinman H A,Hoffman L C.A high incidence of species substitution and mislabelling detected in meat products sold in South Africa[J].Food Control,2013,32(2):440-449.
[22] Bánáti D.European perspectives of food safety[J].Journal of the Science of Food and Agriculture,2014,94(10):1941-1946.
[23] Walker M J,Burns M,Burns D T.Horse meat in beef products-species substitution 2013[J].Journal of the Association of Public Analysts,2013,41:67-106.
[24] Kane D E,Hellberg R S.Identification of Species in Ground Meat Products Sold on the US Commercial Market using DNA-Based Methods[J].Food Control,2015,59:158-163.
[25] 谢莉萍,徐广锐,谢军,等.应用PCR方法鉴别猪肉和牛肉[J].生物学通报,2015,50(6):47-49. [26] 梁慧珍,崔丽华,张春辉,等.PCR-DGGE技术及其在食品行业中的应用[J].酿酒科技,2008,10:89-91. [27] Satokari R M,Vaughan E E,Akkermans A D L,et al.Bifidobacterial diversity in human feces detected by genusspecific PCR and denaturing gradient gel electro phoresis[J].Applied and Environmental Microbiology,2001,67(2):504-513.
[28] 朱燕,罗欣,徐幸莲,等.中国黄牛背最长肌中capnl mRNA表达与嫩度的关系[J].南京农业大学学报,2006,29(2):89-93. [29] Safdar M.Multiplex analysis of animal and plant species origin in feedstuffs and foodstuffs by modern PCR techniques:Qualitative PCR and real time PCR[D].Turkey:Fatih University,2013.
[30] Heid C A,Stevens J,Livak K J,et al.Real time quantitative PCR[J].Genome Research,1996,6(10):986-994.
[31] Safdar M,Junejo Y.A multiplex-conventional PCR assay for bovine,ovine,caprine and fish species identification in feedstuffs:Highly sensitive and specific[J].Food Control,2015,50:190-194.
[32] Kitpipit T,Sittichan K,Thanakiatkrai P.Direct-multiplex PCR assay for meat species identification in food products[J].Food Chemistry,2014,163:77-82.
[33] Hird H,Chisholm J,Sánchez A,et al.Effect of heat and pressure processing on DNA fragmentation and implications for the detection of meat using a real-time polymerase chain reaction[J].Food Additives and Contaminants,2006,23(7):645-650.
[34] Primrose S,Woolfe M,Rollinson S.Food forensics:methods for determining the authenticity of foodstuffs[J].Trends in Food Science&Technology,2010,21(12):582-590.
[35] Smith V S.DNA barcoding:perspectives from a’Partners hips for E nhancing Expertise in Taxonomy’(PEET)debate[J].Syst Biol,2005,54:841-844.
[36] Hebert P D N,Penton E H,Burns J M,et al.Ten species in one:DNA barcoding reveals cryptic species in the neotropical skipper butterfly A straptes fulgerator[J].Proc Natl Acad Sci USA,2004,101:14812-14817.
[37] 张宇,郭良栋.真菌DNA条形码研究进展[J].菌物学报,2012,31(6):809-820. [38] Ali M A,Gyulai G,Hidvegi N,et al.The changing epitome of species identification-DNA barcoding[J].Saudi Journal of Biological Sciences,2014,21(3):204-231.
[39] Edwards D,Horn A,Taylor D,et al.DNA barcoding of a large genus,Aspalathus L.(Fabaceae)[J].Taxon,2008,57(4):1317.
[40] Gregory T R.DNA barcoding does not compete with taxonomy[J].Nature,2005,434(7037):1067.
[41] Schindel D E,Miller S E.DNA barcoding a useful tool for taxonomists[J].Nature,2005,435(7038):17.
[42] Ebach M C,Holdrege C.DNA barcoding is no substitute for taxonomy[J].Nature,2005,434(7034):697.
[43] Seberg O,Humphries C J,Knapp S,et al.Shortcuts in systematics?A commentary on DNA-based taxonomy[J].Trends in Ecology&Evolution,2003,18(2):63-65.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
