Modelling and the induced resistance of inactivation of Staphylococcus aureus to high hydrostatic pressure incubated at different culture temperatures
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摘要: 探讨不同培养温度处理诱导金黄色葡萄球菌(ATCC 6538)对超高压的抗性,并建立不同培养温度下金黄色葡萄球菌的超高压抗性模型。金黄色葡萄球菌经不同温度培养,在100~500 MPa的超高压条件下,选用线性、Weibull和Gompertz三种模型来拟合超高压抗性曲线,以决定系数(R2),均方误差(RMSE),精确因子(Af)和偏差因子(Bf)作为模型拟合度优劣的评判指标。实验结果表明,在100~500 MPa压力的作用下,线性模型的拟合效果不佳,R2最小值达到0.8870,Weibull和Gompertz模型对超高压的抗性具有较好的拟合性(R2≥0.9467),且Weibull模型的拟合效果最好,R2最大值达到0.9956,RMSE最小值为0.0312。因此,Weibull模型可以很好地拟合金黄色葡萄球菌以不同的培养温度胁迫后在超高压作用下的抗性曲线,随着培养温度的升高,金黄色葡萄球菌的超高压抗性呈增加趋势。Abstract: The resistance models of Staphylococcus aureus( ATCC6538) were established in order to investigate the development of resistance responses to high hydrostatic pressure( HHP) treatment in S.aureus cultured at different temperatures. The resistance data for S. aureus incubated at different temperatures were obtained under the conditions of the pressures from 100 to 500 MPa. Then Linear,Weibull,and Gompertz models were selected to fit the curves of resistance to HHP of S. aureus. Regression coefficients( R2),root mean square error( RMSE),accuracy factor( Af) and bias factor( Bf) were used to evaluate the goodness of fit for the three models. Results showed that the Linear model provided a poor fit for the data,which has minimum R2( 0.8870),and Weibull model and Gompertz model showed suitable goodness- of- fit at five pressure levels between 100 to 500 MPa( R2≥0.9467).The Weibull model,which had maximum R2( 0.9956) and minimum RMSE( 0.0312),could be fitted perfectly to the curves of resistance to HHP in the three models.In conclusion,Weibull model showed ideal goodness- of- fit to the curves of resistance to HHP inactivation of Staphylococcus aureus. Moreover,with increasing incubation temperatures,the pressure resistance of S.aureus increased.
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Keywords:
- high hydrostatic pressure(HHP) /
- Staphylococcus aureus /
- Weibull model
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