Determination of ethanol content in fruits by gas chromatography
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摘要: 以樱桃、草莓、梨、枣四种水果为试材,采用DB-WAX（60 m×0.25 mm×0.25μm）毛细管色谱柱和氢火焰离子化检测器建立了气相色谱法测定水果中乙醇含量的方法。结果表明,在进样口温度230℃,FID检测器温度300℃,程序升温40℃（保持11 min）,以20℃/min升至220℃（保持13 min）,以氮气为载气,柱流量2 m L/min的条件下,乙醇有良好的分离效果。乙醇含量在0.25100μg/m L范围内,具有良好的线性关系（R2=0.99998）,最低检出浓度为1.25 mg/kg。该方法稳定性、重复性和重现性良好,RSD均小于2%。在四种水果低中高三个添加水平下,平均回收率在90.88%108.80%之间（RSD=0.18%7.18%,n=6）。该方法操作简单,准确可靠,符合方法学要求,可用于水果中乙醇含量的检测。Abstract: The ethanol content in fruits( cherry,strawberry,pear,jujube) were determined by gas chromatography( GC) equipped with DB-WAX( 60 m × 0.25 mm × 0.25 μm) and hydrogen flame ionization detector.Results showed that the inlet and FID detector temperature were 230 ℃ and 300 ℃,respectively.The column temperature program was initial 40 ℃( held for 11 min),with the increase rate of 20 ℃ / min to 220 ℃( held for 13 min). The carrier gas was nitrogen,and the flow rate was 2 m L / min. The calibration curve showed good linearity when ethanol concentration was in the range of 0.25~100 μg / m L with correlation coefficient of 0.99998,and the detection limit was1.25 mg / kg.The method was stable,repeatable and reproducible with RSD less than 2%. The average recoveries ranged from 90.88% to 108.80%( RSD = 0.18% ~ 7.18%,n = 6). The method was simple,accurate,and met the requirements of methodology,which may be used for the determination of the ethanol in fruits.
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Keywords:
- ethanol /
- fruit /
- chromatography(GC)
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[1] 樊娟,刘芳春.红元帅苹果贮藏过程中乙醇含量的测定[J].中国卫生检验杂志,2012,22(9):2061-2062,2065. [2] Pesis E,Dvir O,Feygenberg O,et al.Production of acetaldehyde and ethanol during maturation and modified atmosphere storage of litchi fruit[J].Postharvest Biology and Technology,2002,26(2):157-165.
[3] Zhang C Y,Lin N B,Chai X S,et al.A rapid method for simultaneously determining ethanol and methanol content in wines by full evaporation headspace gas chromatography[J].Food Chemistry,2015,183:169-172.
[4] 陈霞.葡萄酒中乙醇含量的气相色谱法测定[J].中国酿造,2010,(9):152-153. [5] 杜利敏,陈宁宁,袁野.毛细管柱气相色谱法测定白酒中乙醇的浓度[J].医药论坛杂志,2015,36(3):64-65. [6] 孙夏荣,吴馨.气相色谱法测定紫杉醇注射液中乙醇的含量[J].生物技术世界,2012,(5):36-37,39. [7] 赵美艳,徐琛,吕燕平,等.气相色谱法测定聚维酮碘溶液中乙醇和乙酸的残留量[J].中国临床药学杂志,2014,23(2):103-106. [8] 黄姣,马明,陈丹超,等.气相色谱法测定香水中乙醇含量的研究[J].分析科学学报,2012,28(6):874-876. [9] 霍晓敏.气相色谱内标法测定化妆品中的甲醇和乙醇含量[J].日用化学品科学,2012,35(8):27-29. [10] 黄艳凤,王永婵,张平.静态顶空气相色谱法快速测定葡萄中乙醇和乙醛含量的研究[J].保鲜与加工,2009,9(6):28-30. [11] 纪淑娟,尹竞男,李家政,等.静态顶空气相色谱法测定蜜柚中乙醇和乙醛含量[J].保鲜与加工,2010,10(4):17-20. [12] 赵玉梅,龙柳燕,丁薪源,等.静态顶空气谱法同时测定李果实乙醇及乙醛含量[J].食品研究与开发,2013,34(5):80-83. [13] Burdon J,Lallu N,Yearsley C,et al.The kinetics of acetaldehyde and ethanol accumulation in‘Hass’avocado fruit during induction and recovery from low oxygen and high carbon dioxide conditions[J].Postharvest Biology and Technology,2007,43(2):207-214.
[14] 张艳艳,陈恺,许铭强,等.气相色谱法测定红枣中乙醛、乙醇的含量[J].包装与食品机械,2012,30(5):13-15,19. [15] 曹建康,姜微波,赵玉梅.果蔬采后生理生化实验指导[M].中国轻工业出版社,2007. [16] GB/T 27404-2008实验室质量控制规范食品理化检测[S].北京:中华人民共和国卫生部,2008.
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