Effects of Ca2+ on the growth and content of photosynthetic pigment of Arthrospira
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摘要: 为探讨Ca2+对节旋藻生物量、叶绿素、类胡萝卜素及藻胆蛋白含量的影响,本实验以节旋藻TJSD091藻株为研究对象,在AB液体培养基中加入不同浓度的Ca2+,在TJSD091藻株的快速生长期末测定生物量、叶绿素、类胡萝卜素和藻胆蛋白的含量。结果表明:当Ca2+浓度在0.02 g/L时藻蓝蛋白和别藻蓝蛋白的浓度最高,分别为7.607、5.608 mg/L;当Ca2+浓度在0.06 g/L时生物量、叶绿素、类胡萝卜素含量较低,分别为A560 nm=0.789、5.149、0.193 mg/L;当Ca2+浓度在0.08 g/L时生物量、叶绿素和类胡萝卜素含量处于较高值,分别为A560 nm=1.193、16.13、0.257 mg/L。方差分析表明,Ca2+浓度对节旋藻TJSD091藻株的生长及光合色素含量均有显著影响（p<0.05）。Abstract: To investigate the effect of Ca2+ on biomass,the content of chlorophyll,carotenoid and phycobiliproteins of Arthrospira, Arthrospira strain TJSD091 was taken as the research object in this experiment,different concentrations of Ca2+ was added in AB liquid culture medium respectively,and the biomass,the content of chlorophyll,carotenoid and phycobiliproteins at the end of the rapid growth period of Arthrospira were determined.The results showed that when the concentration of Ca2+ was 0.02 g/L,the PC and APC were the highest,which were 7.607 mg/L and 5.608 mg/L respectively.When the concentration of Ca2+ was 0.06 g/L,the biomass,content of chlorophyll and carotenoid were relatively low,which were A(560 nm)= 0.789,5.149,0.193 mg/L respectively.When the concentration of Ca2+ was 0.08 g/L,the biomass,content of chlorophyll and carotenoid were relatively high,which were A(560 nm)= 1.193,16.13,0.257 mg/L respectively.The variance analysis showed that the concentration of Ca2+ had a significant effect on the biomass and the contents of chlorophyll,carotenoid and phycobiliprotein( p < 0.05).
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Keywords:
- Ca~(2+) /
- Arthrospira /
- biomass /
- chlorophyll /
- carotenoid /
- phycobiliprotein
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