Analysis of physicochemical parameters and bacteria community structure in the Koumiss' s fermentation process
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摘要: 分析了酸马奶发酵过程中(0~96 h)部分理化指标以及细菌群落的变化情况。结果显示酸马奶发酵96 h内p H为下降趋势,发酵初期迅速酸化,发酵后期下降速度缓慢趋于稳定,最低达到p H3.54;总蛋白质、脂肪含量存在一定的起伏变化,72 h达到最高值;乳糖含量减少,而乳酸含量增加;葡萄糖、半乳糖、乙酸、丙酸含量在发酵过程中处于较低的水平;细菌群落结构分析结果显示酸马奶发酵过程中存在丰富的细菌多样性,并且群落结构有明显的演替变化。乳杆菌属(Lactobacillus)为优势细菌属,在酸马奶发酵过程中12~24 h呈现出上升的趋势,而24~48 h时其含量下降。研究结果表明不同发酵时间酸马奶营养价值存有差异。Abstract: To analyze the physicochemical parameters and bacteria community structure in the Koumiss 's fermentation process( 0 ~ 96 h). The main findings were introduced in following part. During the fermentation process,the p H value of Koumiss was declined at high speed in early period and then the decent speed was slowed down and finally reached to the minimum value( p H = 3.54). The total contents of protein and fats were fluctuant changed,and achieved to the maximum values at 72 h. The content of lactose was decreased,while the lactic acid was increased in the process of fermentation. The contents of glucose,galactose,acetic acid,and propionic acid were at low level during fermentation.The results of bacterial community structure analysis showed that there were abundant bacterial diversity and their dynamic community structures changed obviously in the Koumiss fermentation process. Lactobacillus was the dominant bacterial genus. Its content showed a rising trend during 12 h to 24 h,while decling at 24 h to 48 h.The result showed that there were differences in nutritional value of Koumiss at different fermentation period.
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[1] 刘亚东.马奶营养价值评定及在早产儿配方乳中的应用[D].哈尔滨:东北农业大学,2012. [2] Miraglia N,Martin-Rosset W.Nutrition and feeding of the broodmare[M].Wageningen,The Netherlands:Wageningen Academic Publishers,2006:133-147.
[3] 刘洪元,安建钢,王海英,等.鲜马奶缓解体力疲劳的研究[J].中国乳品工业,2010,38(2):24-27. [4] 殷文政,乌尼,钱建伟.锡林郭勒牧区马奶酒生物活性动态的研究[J].内蒙古农业大学学报,2002,23(1):9-16. [5] 孙天松,王俊国,张列兵.中国新疆地区酸马奶中乳酸菌生物多样性研究[J].微生物学通报,2007,34(3):451-455. [6] 乌日娜.内蒙古传统酸马奶中乳杆菌的分离鉴定及16S r DNA序列同源性分析[D].呼和浩特:内蒙古农业大学,2005. [7] 孟和毕力格,乌日娜,王立平.不同地区酸马奶中乳杆菌的分离及其生物学特性的研究[J].中国乳品工业,2004,32(11):6-11. [8] Ying A N,Adachi Y,Ogawa Y.Classification of lactic acid bacteria isolated from chigee and mare milk collected in Inner Mongolia[J].Animal Science Journal,2004,75(3):245-252.
[9] 熊素玉,姚新奎,谭小海,等.酸马奶中乳酸菌的分离、纯化与鉴定[J].新疆农业科学,2007,44(5):696-701. [10] 霍小琰,李少英,郭荣荣.酸马奶中乳酸菌的鉴定及生物学特性的研究[J].微生物学通报,2012,39(7):940-948. [11] 刘芳,都立辉,杜鹏,等.内蒙古酸马奶中乳酸菌多样性的研究(英文)[J].食品科学,2008,29(2):218-224. [12] Hao Y,Zhao L,Zhang H.Identification of the bacterial biodiversity in koumiss by denaturing gradient gel electrophoresis and species-specific polymerase chain reaction[J].Journal of Dairy Science,2010,93(5):1926-1933.
[13] GB 5009.5—85,食品中蛋白质的测定方法,中国标准出版社,1988. [14] 黄伟坤.食品检验与分析[M].北京:中国轻工业出版社,1999,421-422. [15] 梁龙,牟光庆,陈历俊,等.高效液相色谱法测定混菌发酵乳中乳糖,葡萄糖及半乳糖含量的变化[J].食品工业科技,2013,34(13):292-295. [16] Lee OO,Wang Y,Yang J,et al.Pyrosequencing reveals highly diverse and species specific microbial communities in sponges from the Red Sea[J].ISME J,2011,5(4):650-664.
[17] Kuczynski J,Stombaugh J,Walters W A,et al.Using QIIME to Analyze 16S rRNA Gene Sequences from Microbial Communities[J].Curr Protoc Microbiol,2012,Chapter 1,Unit1E 5.
[18] Cole J R,Chai B,Farris R J,et al.The ribosomal database project(RDP-II):introd-ucing my RDP space and quality controlled public data[J].Nucleic Acids Res,2007,35(Database issue):169-172.
[19] 孙健.酸马奶酒中抗菌物质的分离及抗菌作用的研究[D].呼和浩特:内蒙古农业大学,2003. [20] 崔玉琦,王雅炜,武瑞霞,等.酵母菌在发酵乳制品中的应用研究[J].食品科技,2014(10):53-56. [21] 孙天松.新疆维吾尔自治区酸马奶化学组成分与微生物学分析[J].中国乳品工业,2005,33(10):9-13.
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