基于响应曲面法的中老年营养米粉酶解工艺优化研究
详细信息Enzymatic hydrolysis technology optimization of the nutritious rice cereal for mid- aged and older people based on response surface methodology
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摘要: 以优质碎米为原料,利用单因素实验、Box-Behnken实验设计和响应面分析研究浆料浓度、酶解时间、酶解温度、酶添加量对水解程度（DE值）的影响,优化酶解工艺参数,结合喷雾干燥技术,制得中老年营养米粉基料。优化结果表明,在浆料浓度13%、酶解时间42 min、酶解温度69℃、酶添加量0.34%条件下生产的米粉基料水解程度可达33.02%,米粉中无残存酶活力,淀粉消化指数比非酶解工艺提高21.71%,达到50.62%。Abstract: Using the high quality broken rice as raw material,the effect of substrate concentration,enzymatic hydrolysis time,enzymatic hydrolysis temperature and enzyme concentration on the degree of hydrolysis( DE value) was systematically studied by single factor experiments. Box- Behnken design and response surface analysis,then the enzymatic hydrolysis parameters were optimized,and the basic material of mid- aged and older nutritious rice cereal was prepared combined with spray drying technology.In the conclusion,the optimal hydrolysis conditions were established as follows: substrate concentration 13%,enzymatic hydrolysis time 42 min,temperature69 ℃,enzyme concentration 0.34%. Under the optimum enzymatic hydrolysis conditions,the dextrose equivalent reached to 33.02%,moreover,there was no residual enzyme activity,and the starch digestion index was 50.62%,which was higher compared to the non- enzymatic productions by 21.71%.
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