Study on the antioxidant and inhibiting non-enzyme glycation activities of crude flavonoids from wheat germ in vitro
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摘要: 采用自由基清除体系(DPPH、羟基及超氧阴离子)、螯合金属离子能力、还原能力及总抗氧化能力体系评价麦胚黄酮粗提物的体外抗氧化活性,并考察麦胚黄酮粗提物对体外非酶糖基化反应的抑制作用。结果表明,麦胚黄酮粗提物清除自由基的能力、还原能力、总抗氧化能力均随浓度的增大而增强,且清除DPPH自由基、羟基自由基能力的IC50值分别为0.26、0.78 mg/m L。在浓度0.75~3.0 mg/m L之间,随浓度增大,其金属离子螯合能力逐渐增强至78.60%,继续增大浓度至3.75 mg/m L时,螯合能力降低至70.31%,但强于VC。此外,麦胚黄酮粗提物对体外蛋白质非酶糖基化终末产物(AGEs)的形成有抑制作用,且随浓度增大,抑制效果逐渐增强。1.0 mg/m L麦胚黄酮粗提物对AGEs生成的抑制率在第7 d可达到48%。综上可见,具有强体外抗氧化能力的麦胚黄酮,能有效抑制AGEs的生成。Abstract: In this paper,the antioxidant activities of crude flavonoids from wheat germ( CFWG) in vitro were determined by radical scavenging system( DPPH radicals,hydroxyl radicals,superoxide anion),metal ion chelating ability,reducing power and total antioxidant capacity system. In addition,the inhibiting non- enzymatic glycation reaction of CFWG was also evaluated. The results showed that,with the increasing of concentration,the antioxidant activities of CFWG,such as scavenging DPPH radicals,hydroxyl radicals and superoxide anion radicals ability,and reducing power,total antioxidant capacity were increased. The IC50 values of scavenge DPPH radical and hydroxyl radicals were 0.26,0.78 mg / m L,respectively. In concentration of 0.75 ~ 3.0 mg / m L,with the concentration increased,its metal ion chelating ability was gradually increased to 78.60%,and when the concentration was 3.75 mg / m L,its metal ion chelating ability was reduced to 70.31%,which was also stronger than VC.Furthermore,CFWG can inhibit the advanced glycation end products( AGEs) in vitro,and with the concentration increasing,the inhibitory effect was gradually increasd. In the first seven days,the inhibition rate of 1.0 mg / m L CFWG was up to 48%.In a word,CFWG with strong antioxidant capacity in vitro,can inhibit the formation of AGEs effectively.
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