Effect of Collybia radiata polysaccharides on intestinal flora and secretory immunoglobulin A in mice
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摘要: 为了研究长根菇多糖对小鼠的肠道菌群及肠黏膜分泌型免疫球蛋白A(secretory immunoglobulin A,SIg A)的影响,选取18~22g健康清洁级雄性昆明小鼠进行肠道菌群和SIg A的检测。利用盐酸林可霉素灌胃建立肠道微生态失调小鼠模型。60只小鼠随机分为五组,正常对照组、自然恢复组、低剂量多糖组(75mg/kg·bw)、中剂量多糖组(150mg/kg·bw)和高剂量多糖组(300mg/kg·bw)。灌胃14d后分别进行肠道双歧杆菌、乳酸杆菌、大肠杆菌、肠球菌检测和肠黏膜SIg A测定。结果显示,与空白对照组比较,其余各组均有双歧杆菌、乳酸杆菌、SIg A减少以及大肠杆菌、肠球菌增多的现象;与自然恢复组比较,多糖组上述指标得到明显改善,差异显著(p<0.05)。结果表明,长根菇多糖对肠道菌群失调小鼠的肠道菌群具有调整作用并能提高肠黏膜SIg A含量。Abstract: To investigate the effect of Collybia radiata polysaccharide on intestinal flora and secretory immunoglobulin A ( SIg A) of mouse, the detection of intestinal flora and SIg A were carried out on the mice kunming mice.Lincomycin hydrochloride intragastric administration was used to construct mouse intestinal dysbacteriosis model.Sixty mice were randomly put into one standard control group, one spontaneous recovery group and three different doses of Col ybia radiata polysaccharide treatment groups, fed polysaccharide at dosage of 75, 150 mg / kg·bw, and 300 mg / kg·bw, respectively. After being treated for 14 days, the counts of Bifidobacterium, Lactobacillus, Escherichia coli, Enterococcus and SIg A were assayed. The results showed that compared with the standard control group, intestinal microflora was disturbed and the content of intestinal mucosal SIg A was significantly decreased in the other four groups. And compared with the spontaneous recovery group, the above indexes renewed to some extent.The results indicated that Collybia radiata polysaccharide has played a role in adjusting intestinal flora and improving content of SIg A.
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Keywords:
- Collybia radiata /
- polysaccharide /
- mice /
- intestinal flora /
- secretory immunoglobulin A
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