Anti- fatigue effects of polysaccharides from Hericium erinaceus in mice
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摘要: 研究猴头菇多糖的抗疲劳作用及单糖组成。分别以猴头菇粗多糖(Crude polysaccharide from Hericium erinaceus,HEPC)、中性多糖(Neutral polysaccharide from Hericium erinaceus,HEPN)、酸性多糖(Acidic polysaccharide from Hericium erinaceus,HEPA)和蒸馏水灌胃小鼠,连续灌胃15d后进行负重游泳实验,考察其对力竭游泳时间及血乳酸(Blood lactic acid,BLA)、血清尿素氮(Blood urea nitrogen,BUN)、肝糖原(Hepatic glycogen,HG)含量的影响。结果显示猴头菇多糖,特别是酸性多糖能显著延长小鼠负重游泳时间,提高小鼠体内肝糖原的储备量,降低小鼠血乳酸和血清尿素氮含量。该结果表明猴头菇多糖对小鼠具有抗疲劳作用,且猴头菇酸性多糖的作用最强。将猴头菇多糖水解和PMP衍生化后,用高效液相色谱法(High performance liquid chromatography,HPLC)分析其单糖组成,显示猴头菇多糖是由甘露糖、鼠李糖、葡萄糖、半乳糖、岩藻糖和葡萄糖醛酸组成的杂多糖。Abstract: To investigate the anti- fatigue effects of polysaccharides from Hericium erinaceus ( HEP) and monosaccharide composition.Mice were orally administrated with Crude polysaccharide from Hericium erinaceus ( HEPC) , Neutral polysaccharide from Hericium erinaceus ( HEPN) , Acidic polysaccharide from Hericium erinaceus ( HEPA) and distilled water. After 15 days, all mice were subjected to weight- loaded swim test. The exhaustive swimming time, blood lactic acid, blood urea nitrogen and hepatic glycogen were measured. The results showed that HEP especially HEPA could significantly lengthen the swimming time of mice, improve the mice hepatic glycogen reserves, reduce blood lactic acid and blood urea nitrogen content. The HEP has anti- fatigue function and the strongest was HEPA.After PMP derivatization, High performance liquid chromatography ( HPLC) was used for analyzing monosaccharide composition of polysaccharides. The result indicated that HEP was heteropolysaccharide and consisted of mannose, rhamnose, glucose, galactose, fucose and glucuronic acid.
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