Identifying the authenticity and producing area of panax using non- linear chemical fingerprint
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摘要: 利用非线性化学指纹图谱对7个不同产地的人参及其伪品以及其它参类进行研究,提出鉴别人参真伪和产地的新方法。向"硫酸-硫酸锰-丙酮-溴酸钠"混合液中加入样品,产生非线性化学反应,通过优化反应条件,检测不同产地和种类样品的指纹图谱,并确定其系统相似度。结果表明:样品用量为0.6g,反应温度为37℃时,样品非线性化学指纹图谱具有良好重现性和特征性。不同种类的样品具有明显不同特征的指纹图谱;同一产地人参的系统相似度在0.97120.9920之间,而不同产地人参的相似度在0.75480.9403范围内。利用人参非线性化学指纹图谱的直观特征和系统相似度可分别准确地鉴定人参的真伪和产地。Abstract: Samples of panax from 7 producing areas and its fakes as well as other ginsengs were investigated using non- linear chemical fingerprint, and a new method to identify the authenticity and producing area of panax sample was put forward. Nonlinear chemical reactions could took place in the systems consisting of H2SO4, Mn SO4, CH3COCH3, Na Br O3 and components of ginseng samples and the optimal reaction conditions were investigated by adjusting the sample dosage and reaction temperature. The fingerprints of samples of different kinds and producing areas were determined under the optimal conditions, and their system similarities were determined.The results showed that when dosage of detection was 0.6g and the reaction temperature was 37℃, the non- linear chemical fingerprints had good reproducibility and characteristic. The fingerprint characteristics of different ginsengs were obviously different; the system similarities of panax samples from the same producing area were between 0.9712 ~ 0.9920, whereas those from different producing areas were 0.7548 ~ 0.9403. The authenticity and producing area of panax could be identified accurately by the visual characteristic and system similarity of its non- linear chemical fingerprint, respectively.
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Keywords:
- panax /
- non-linear chemical fingerprint /
- identification /
- authenticity /
- producing area
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[1] 李好枝.HPLC法鉴别西洋参和人参[J].沈阳药科大学学报, 1997, 14 (2) :124-125. [2] 赵亮, 吕磊.高效液相色谱法快速测定人参中8种主要皂苷类成分的含量[J].第二军医大学学报, 2008, 29 (12) :1507-1510. [3] 高阳, 袁永越.人参药材色谱指纹图谱分析方法研究[J].中成药, 2006, 28 (4) :473-477. [4] 张萍, 张南平.人参皂苷类成分的化学成分[J].药物分析杂志, 2004, 24 (3) :229-237. [5] 肖新月.不同生长年限的人参中8种主要皂苷成分的分析研究[J].药物分析杂志, 2004, 24 (3) :238-244. [6] 齐滨, 刘莉.人参、西洋参和红参中糖类含量的比较研究[J].中国药房, 2013, 24 (7) :616-618. [7] 王丹, 吕永磊.人参多糖含量测定方法研究[J].中华中医药杂志, 2011, 26 (4) :774-776. [8] 李家旺.用傅里叶变换红外光谱法研究核辐射对人参粉成分的影响[J].光散射学报, 2011, 23 (1) :83-89. [9] 朱萱萱.气相色谱法测定人参中残留有机氯农药的含量[J].中国药学杂志, 1997, 32 (8) :484-486. [10] Zhang T M, Liang Y Z, Yuan B, et al.Determining method and conditional factors of electrochemical fingerprint of Chinese traditional medicine[J].Chinese Science Bulletin, 2007, 52 (16) :2190-2202.
[11] Zhang T M, Liang Y Z, Yuan B, et al.Principle, traits and functions of electrochemical fingerprint for distinguishing and evaluating Chinese traditional medicine[J].Chinese Science Bulletin, 2007, 52:2753-2765.
[12] Zhang T M, Zhao Z, Fang X Q, et al.Determining method, conditional factors, traits and applications of non-linear chemistry fingerprint by using dissipative components in samples[J].Science China Chemistry, 2012, 55:285-303.
[13] Zhang, T M, Zhao Z, Fang X Q, et al.Principle of non-linear chemistry fingerprint by using dissipative components in samples and calculation-and-evaluation of non-linear chemical fingerprint similarity[J].Science China Chemistry, 2012, 55:304-322.
[14] Fang X Q, Zhang T M, Zhao Z, et al.Application of nonlinear chemical fingerprinting to identification, evaluation and clinical use of Glycyrrhiza[J].Chinese Science Bulletin, 2010, 55:2937-2944.
[15] 张娟, 钟俊辉, 王志沛, 等.利用非线性化学指纹图谱法测定啤酒中乙醇含量[J].分析化学, 2014, 42:559-564. [16] 张海珍, 肖长龙, 杨学华, 等.非线性化学指纹图谱在蜂蜜质量评价中的应用[J].食品研究与开发, 2012, 33 (4) :194-197. [17] 杨代明, 方宣启, 冯敏, 等.食醋非线性化学指纹图谱掺伪鉴别原理的研究[J].食品和机械, 2012, 28 (1) :86-92.
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