葡萄赭曲霉毒素污染及其产毒素菌株的筛选方法研究进展

王海英; 张红印; 杨其亚; 李超兰; 王俊青; 刘正伟

doi:10.13386/j.issn1002-0306.2015.10.070

葡萄赭曲霉毒素污染及其产毒素菌株的筛选方法研究进展

1. 江苏大学食品与生物工程学院
2. 河南永达食业集团技术中心

基金项目:

国家自然科学基金(31271967)；教育部高等学校博士学科点专项科研基金(20123227110015)；镇江市科技支撑计划-农业支撑项目(NY2013020NY2013004)；

详细信息

作者简介:
王海英 (1988-) , 女, 硕士研究生, 研究方向:食品生物技术。;

张红印 (1972-) , 男, 博士, 教授, 研究方向:食品生物技术。;

中图分类号: TS201.3
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- 收稿日期: 2014-07-20

Advance of research on ochratoxin contamination in grapes and screening methods of toxin producing strains

College of Food and Biological Engineering, Jiangsu University
Food Group Technology Center

摘要

摘要: 赭曲霉毒素A(ochratoxin,OTA)是由曲霉属和青霉属等真菌产生的一类真菌毒素,其毒性很强,分布广泛,对人类和动植物的健康有着巨大的影响。葡萄及其制品是食品中OTA的主要来源之一,从病害的葡萄表面筛选分离产生赭曲霉毒素的菌株是最常用的研究产毒素菌株的方法。由于产毒素菌株主要分布在葡萄果实的表面,葡萄组织受损后会极大提高赭曲霉素的污染程度,研究产毒菌株分布及产毒素能力对控制赭曲霉素污染及寻找一种有效地生物防治方法提供参考。本文综述了葡萄赭曲霉毒素的污染情况及其产毒素菌株的筛选方法,为控制葡萄及其产品中的OTA污染提供依据。
- 赭曲霉毒素A /
- 筛选 /
- 控制 /
- 检测
Abstract: Ochratoxin A (OTA) was a toxic secondary metabolite produced by Aspergillus and Penicillium fungi.OTA had a huge impact on human and animal health due to its strong toxicity and wide distribution. Grape and its products were one of the main sources of food for OTA. Isolation and screening of strains producing ochratoxin from the surface of diseased grapes was the most commonly used method to research toxigenic strains. Because the toxigenic strains were mainly distributed in the the surface of grapes, theochratoxin contamination levels would be greatly increased when the grape tissue was damaged. Study the distribution of toxigenic strains and its ability of producing toxins provides reference for controlling ochratoxin pollution and finding an effective biological control method. This paper reviewed the occurrence of ochratoxin A (OTA) contamination in grape and the screening methods of mycotoxin producing strains, which provided the basis for the control of OTA pollution in the grape and its products.
- ochratoxin A /
- screening /
- control /
- detection

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参考文献(13)

[1]	杨超.四种真菌毒素检测方法的建立以及赭曲霉毒素A脱除方法的初步研究[D].青岛:中国海洋大学, 2009.
[2]	高翔, 李梅.赭曲霉毒素A的毒性研究进展[J].国外医学卫生学分册, 2005, 32 (1) :51-55.
[3]	Angelo V, Michelangelo P, Gianluca C.Determination of ochratoxin A in domestic and imported beers in Italy[J].Journal of Chromatography A, 2000, 88 (8) :321-326.
[4]	Brera C, Miwaglia M, Colatosti M.Evaluation of the impact exotoxins on human health of source of errors[J].Journal of Agriculture and Food Chemistry, 2002, 50 (25) :7493-7496.
[5]	章英, 许杨.谷物类食品中赭曲霉毒素A分析方法的研究进展[J].食品科学, 2006, 27 (12) :767-771.
[6]	杨家玲, 岳田利, 高振鹏, 等.赭曲霉毒素A检测方法的研究进展[J].农产品加工 (学刊) , 2008, 6:4-7.
[7]	Ponsone M L, Chiotta ML, Combina M, et al.Biocontrol as a strategy to reduce the impact of ochratoxin A and Aspergillus section Nigri in grapes[J].International Journal of Food Microbiology, 2011, 151 (1) , 70-77.
[8]	高翔, 李梅, 张立实.赭曲霉毒素A的毒性研究进展[J].国外医学 (卫生学分册) , 2005, 32 (1) :51-55.
[9]	蒋春美, 师俊玲, 刘延琳.赭曲霉毒素产生菌筛选方法的对比与优选[J].西北农林科技大学学报, 2012, 40 (3) :169-174.
[10]	梁志宏.粮食中赭曲霉毒素A的检测及产毒素菌株的分析与研究[D].北京:中国农业大学, 2008.
[11]	Serge K, Franoise SM, Regine S, et al.Fungal Flora and Ochratoxin A Production In Various Food and Feed in France[J].System Appl Microbiol, 1995, 18, 455-459.
[12]	Timperio A M, Magro P, Chilosi G, et al.Assay of ochratoxin A in grape by high-pressure liquid chromatography coupled on line with an ESI-mass spectrometry[J].Journal of Chromatography B, 2006, 832:127-133.
[13]	疏秀林, 施庆珊, 欧阳友生.赭曲霉毒素的产生及鉴别[J].中国卫生检验杂志, 2008, 18 (10) :2183-2185.

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葡萄赭曲霉毒素污染及其产毒素菌株的筛选方法研究进展

作者简介: 王海英 (1988-) , 女, 硕士研究生, 研究方向:食品生物技术。; 张红印 (1972-) , 男, 博士, 教授, 研究方向:食品生物技术。;

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Advance of research on ochratoxin contamination in grapes and screening methods of toxin producing strains

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目录

作者简介:
王海英 (1988-) , 女, 硕士研究生, 研究方向:食品生物技术。;

张红印 (1972-) , 男, 博士, 教授, 研究方向:食品生物技术。;