Antioxidant activities of tilapia (Oreochromis niloticus) protein and component proteins hydrolysates
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摘要: 采用胃蛋白酶、中性蛋白酶、Alcalase2.4L碱性蛋白酶分别酶解罗非鱼肉蛋白,研究比较不同蛋白酶在1、2、4、6、8h对罗非鱼肉蛋白的酶解效果及产物的抗氧化活性,结果表明:3种酶的酶解产物都具有一定的抗氧化活性,中性蛋白酶酶解产物的水解度和蛋白回收率最高,胃蛋白酶酶解产物对DPPH自由基和羟自由基的清除效果最好;综合各项指标选择中性蛋白酶对罗非鱼肉的组分蛋白(肌原纤维蛋白、肌浆蛋白、基质蛋白)进行4h酶解,研究比较不同组分蛋白的酶解效果及产物的抗氧化活性,结果表明:组分蛋白中基质蛋白酶解产物的蛋白回收率最高,为89.8%,肌原纤维蛋白酶解产物的水解度最高,为10.1%,肌浆蛋白酶解产物的抗氧化活性最好,羟自由基和DPPH自由基的IC50值分别为12.352mg/m L和3.554mg/m L。Abstract: Pepsin, neutral protease, Alcalase2.4L were used to hydrolyse the tilapia protein, respectively. The effect of hydrolysis and antioxidant activities including 1, 1-diphenyl-2-picrylhydrazine (DPPH·) and hydroxyl radical (·OH) of hydrolysates hydrolysed by different proteases for 1, 2, 4, 6, 8h were studied and compared, the results showed that the hydrolysates of three proteases all had antioxidant activities, the hydrolysate of neutral protease had the highest degree of hydrolysis (DH) and the recovery rate of protein, the hydrolysate of pepsin had the minimum IC50 value of DPPH· and ·OH scavenging ability. Neutral protease was selected to hydrolyse sarcoplasmic protein, myofibrillar protein and stroma protein for 4h, the effect of hydrolysis and antioxidant activities of hydrolysates were studied and compared. The results indicated that the hydrolysate of stroma protein had the highest recovery rate of protein that was 89.8%, the hydrolysate of myofibrillar protein had the highest DH that was 10.1%, the hydrolysate of sarcoplasmic protein had the minimum IC50 value of ·OH and DPPH· scavenging ability that were 12.352mg/m L and 3.554mg/m L.
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Keywords:
- tilapia /
- component protein /
- hydrolysis /
- antioxidant activity
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[1] Dai H N, Zhong JQ, Bomi R, et al.In vitro antioxidant activity of a peptide isolated from Nile tilapia (Oreochromis niloticus) scale gelatin in free radical-mediated oxidative systems[J].Journal of Functional Foods, 2010, 2 (2) :107-117.
[2] Samaranayakaa G P, Li-chan E C Y.Autolysis-assisted production of fish protein hydrolysates with antioxidant properties forms pacific hake (Merluccius productus) [J].Food Chemistry, 2008, 107:768-776.
[3] Da YZ, Tang Y, Zhu BW, et al.Antioxidant activity of hydrolysates obtained from scallop (Patinopecten yessoensis) and abalone (Haliotis discus hannai Ino) muscle[J].Food Chemistry, 2012, 132 (2) :815-822.
[4] You LJ, Zhao MM, Cui C, et al.Effect of degree of hydrolysis on the antioxidant activity of loach (Misgurnus anguillicaudatus) protein hydrolysates[J].Innovative Food Science and Emerging Technologies, 2009, 10 (2) :235-240.
[5] 丁利君, 何颖基, 梁燕.非洲鲫鱼蛋白酶解物的抗氧化研究[J].食品研究与开发, 2009, 30 (11) :1-5. [6] 邓敏, 朱志伟, 欧善堂, 等.利用响应面法优化酶解罗非鱼制备抗氧化肽的研究[J].现代食品科技, 2011, 27 (10) :1242-1245. [7] 尤娟, 罗永康, 沈慧星, 等.酶法制备鲢鱼蛋白抗氧化肽研究[J].渔业现代化, 2010, 37 (3) :42-47. [8] 阎欲晓.罗非鱼活性肽分离及抗氧化能力研究[J].水产科学, 2009, 28 (5) :276-279. [9] 胡晓, 孙恢礼, 李来好, 等.我国酶解法制备水产功能性肽的研究进展[J].食品工业科技, 2012, 33 (24) :410-413. [10] 陈胜军, 李来好, 杨贤庆, 等.罗非鱼综合加工利用与质量安全控制技术研究进展[J].南方水产科学, 2011, 7 (4) :85-90. [11] 唐小丹, 周春霞, 洪鹏志, 等.罗非鱼肉蛋白的分离及其性质研究[J].食品科技, 2011, 36 (6) :156-159, 166. [12] 刘在军, 岑剑伟, 李来好, 等.罗非鱼血液综合利用的研究思路及展望[J].南方水产科学, 2012, 8 (2) :76-80. [13] 韩道财, 洪鹏志, 杨萍.均匀设计法优化罗非鱼肉富肽酶解液制备工艺[J].食品研究与开发, 2012, 33 (1) :155-158. [14] 赵珊珊, 朱志伟, 曾庆孝, 等.不同蛋白酶酶解罗非鱼肉制备蛋白水解液的过程变化规律研究[J].现代食品科技, 2008, 24 (2) :115-119. [15] 马赛蕊, 胡晓, 吴燕燕, 等.罗非鱼肉蛋白酶解液的抗氧化活性[J].食品科学, 2012, 33 (19) :52-56. [16] Hashimoto K, Watabe S, Kono M, et al.Muscle protein composition of sardine and mackerel[J].Bull Jpn Soc Sci Fish, 1979, 45:1435-1441.
[17] Visessanguan W, Benjakul S, Riebroy S, et al.Changes in composition and functional properties of proteins and their contributions to Nham characteristics[J].Meat Sci, 2004, 66:579-588.
[18] Saito T.Effect of thermal on extraction of proteins from meats[J].Bull Jpn Soc Sci Fish, 1983, 46:1569-1597.
[19] 张意静.食品分析技术[M].北京:中国轻工业出版社, 2001:186-194. [20] 朱俭.生物化学实验[M].上海:上海科学技术出版社, 1987:100-159. [21] Jin M, Cai YX, Li JR, et al.1, 10-Phenanthroline-Fe2+oxidative assay of hydroxyl radical produced by H2O2/Fe2+[J].Progress in Biochemistry and Biophysics, 1996, 23 (6) :553-555.
[22] Baea S H, Suh H J.Antioxidant activities of five different mulberry cultivars in Korea[J].LWT-Food Science and Technology, 2006, 40 (3) :955-962.
[23] 朱志伟, 曾庆孝, 林奕封.采用控制酶解法从罗非鱼肉中制备血管紧张素转化酶抑制肽[J].食品与发酵工业, 2004, 30 (12) :71-76. [24] 何婷.蓝园鲹蛋白控制酶解及酶解物抗氧化特性研究[D].广州:华南理工大学, 2008. [25] 李琳, 赵谋明.鳙鱼蛋白酶解液清除自由基的研究[J].水产科学, 2005, 24 (10) :15-18. [26] Shimada K, Fujikawa K, Yahara K, et al.Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion[J].Journal of Agricultural and Food Chemistry, 1992, 40 (6) :945-948.
[27] Bondet V, Brand W W, Berset C.Kinetics and mechanisms of antioxidant activity using the DPPH·free radical method[J].Food Science and Technology, 1997, 30 (6) :609-615.
[28] 杨铭铎, 吴莹莹, 张洪微.不同酶解法水解黑龙江小麦麦胚蛋白的抗氧化功能比较研究[J].食品科学, 2011, 32 (2) :124-126. [29] 任娇艳.草鱼蛋白源抗疲劳生物活性肽的制备分离及鉴定技术研究[D].广州:华南理工大学, 2008.
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