板栗壳中多酚的提取纯化及其抑制α-葡萄糖苷酶活性的研究

基金项目:

国家林业公益性行业科研专项(201204401)； 2012年北京市大学生科学研究与创业行动计划项目(121002221)；

详细信息

Extraction of polyphenols from chestnut shell and the bioactivity of inhibiting α-glycosidase

Department of Food Science and Engineering,College of Biological Science and Technology,Beijing Forestry University
Beijing Key Laboratory of Forest Food Processing and Safety,Beijing Forestry University

摘要: 以板栗壳为原料,经回流提取、浓缩、萃取和大孔树脂吸附洗脱,提取纯化多酚并研究其对α-葡萄糖苷酶的抑制作用。大孔吸附树脂的静态吸附动力学曲线表明,AB-8对板栗壳中多酚的吸附效果明显,达到了4.41mg/g。静态解吸实验表明,当乙醇体积分数60%时解吸的效果最好,能达到95%以上;而pH对解吸的效果影响不大。由动态解吸曲线得到流速对解吸的效果影响较小。多酚洗脱曲线和对α-葡萄糖苷酶抑制率曲线的峰值重叠性较好,说明多酚能有效抑制该酶的活性,抑制率最大达到21.78%。
- 板栗壳 /
- 多酚 /
- α-葡萄糖苷酶 /
- 抑制
Abstract: Polyphenols were extracted and purified by boiling reflux,vacuum concentration,liquid-liquid extraction and macroporous adsorptive resin elution from Chinese chestnut shells,and the inhibiting effect on α-glycosidase was investigated. The static adsorption kinetic curves showed that AB-8 had good adsorption on polyphenols with the capacity of 4.41mg/g. The desorption rate reached above 95% with 60%(v/v) ethanol as eluent. The eluent p H and flow rate had low effects on the desorption rate. There was good overlap between elution curve of polyphenols and inhibition rate,which indicated that polyphenols could inhibit the activity ofα-glycosidase effectively,and the maximal inhibition rate reached 21.78%.
- chestnut shell /
- polyphenol /
- α-glycosidase /
- inhibition