Optimization of extraction process of total phenolic acids from Syringa oblate Lindl. leaves by response surface methodology
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摘要: 采用响应面法对丁香叶总酚酸的乙醇提取工艺进行优化。以总酚酸提取率为考察指标,在单因素实验基础上,采用响应面分析法对乙醇浓度、提取温度、提取时间和液料比等提取条件进行了优化。结果表明,乙醇回流提取总酚酸的最佳工艺条件为:乙醇浓度44%、提取温度90℃、提取时间96min、液料比25.2∶1(m L/g)、提取次数为2次,在此条件下提取率为(1.642±0.23)mg/g。响应面优化得到的提取工艺稳定合理,准确可靠,是提取丁香叶总酚酸的可行方法。Abstract: Total phenolic acids extraction from Syringa oblate Lindl. leaves was performed using the ethanol reflux extraction. Response surface methodology (RSM) , based on a three level, four variable Box-Behnken design ( BBD) , was employed to obtain the best possible combination of ethanol concentration, extraction temperature, extraction time, and water to the raw material ratio for maximum total phenolic acids extraction.The optimum extraction conditions were as follows:ethanol concentration of 44%, extraction time of 96 min, extraction temperature of 90℃, and the ratio of water to raw material of 25.2 ∶1 (m L/g) , extraction times of 2.Under these conditions, the experimental yield was (1.642±0.23) mg/g. The extraction process obtained by response surface methodology was stable and reasonable, accurate and reliable. It was a feasible method to extract the total phenolic acids from Syringa oblate Lindl. Leaves.
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[1] 李博, 朱俊访.丁香叶中所含有效成分的种类及药理作用研究进展[J].黑龙江医药, 2009, 22 (4) :510-511. [2] Blasa M, Candiracci M, Accorsi A, et al.Honey flavonoids as protection agents against oxidative damage to human red blood cells[J].Food Chemistry, 2007, 104 (4) :1635-1640.
[3] 杭亮, 王俊儒, 杨东风, 等.紫花丹参和白花丹参不同部位有效成分的分布特征[J].西北农林科技大学学报, 2008, 36 (12) :218-221. [4] Zhang Y, Li X, Wang ZZ.Antioxidant activities of leaf extract of Salvia miltiorrhiza Bunge and related phenolic constituents[J].Food and Chemical Toxicology, 2010, 48 (10) :2656-2662.
[5] Peter M, Simmonds MS.Rosmarinic acid[J].Phytochemistry, 2003, 62 (2) :121-125.
[6] 黄诒森, 张均田.丹参中3种水溶性成分的体外抗氧化作用[J].药学学报, 1992, 27 (2) :96-100. [7] 孙强, 郭永霞.丁香叶中总黄酮提取工艺及数学模拟[J].中国医院药学杂志, 2013, 33 (14) :1145-1149. [8] 郝婷婷, 严铭铭, 郭婷婷, 等.紫丁香叶中总皂苷提取工艺的研究[J].时珍国医国药, 2008, 19 (6) :1424-1425. [9] 兰掀, 王昶, 朱广伟.丁香叶中丁香酚提取方法的比较[J].黑龙江医药, 2006, 19 (1) :19-20. [10] 米宝丽.中药丁香叶的质量及应用研究[D].沈阳:辽宁中医药大学, 2008. [11] 卫生部药品标准中药成方制剂第九册[S].1998:100. [12] 李富华, 夏春燕, 刘燕妮, 等.响应面法优化蜂胶黄酮提取工艺及其抗氧化活性研究[J].食品工业科技, 2012, 33 (20) :226-230. [13] 刘朝霞, 邹坤, 周媛, 等.酶法预处理苎麻叶提取总酚酸的工艺优化[J].湖北农业科学, 2011, 50 (10) :2105-2107. [14] 张百霞, 郭庆梅, 王真真, 等.响应曲面法优化金银花总酚酸提取工艺[J].中成药, 2013, 35 (10) :2144-2148. [15] 刘荣华, 陈石生, 任刚, 等.星点设计-效应面法优化白茅根总酚酸提取工艺[J].中成药, 2011, 33 (7) :1149-1153. [16] 任凤莲, 谷芳芳, 吴梅林, 等.利用响应面分析法优化山楂中总黄酮提取条件[J].天然产物研究与开发, 2006, 18 (1) :126. [17] Lee WC, Yusof S, Hamid NSA, et al.Optimizing conditions forhotwater extraction ofbanana juice using response surface methodology (RSM) [J].Journal of Food Engineering, 2006, 75 (4) :473-479.
[18] Sin HN, Yusof S, Sheikh AN, et al.Optimization of hot water extraction for sapodilla juice using response surface methodology[J].Journal of Food Engineering, 2006, 75 (3) :352-358.
[19] Wang XS, Wu Q, Wu YF, et al.Response surface optimized ultrasonic-assisted extraction of flavonoids from sparganii rhizoma and evaluation of their in vitro antioxidant activities[J].Molecules, 2012, 17 (6) :6769-6783.
[20] Pan GY, Yu GY, Zhu CH, et al.Optimization of ultrasoundassisted extraction (UAE) of flavonoids compounds (FC) from hawthorn seed (HS) [J].Ultrasonics Sonochemistry, 2012, 19 (3) :486-490.
[21] 胡国华, 陈明.亚麻籽胶的特性及其在冰淇淋中的应用研究[J].食品科学, 2003, 24 (11) :90-93. [22] 国蓉, 李剑君, 国亮, 等.采用响应曲面法优化甘草饮片中甘草酸的超声提取工艺[J].西北农林科技大学学报:自然科学版, 2006, 34 (9) :187-192.
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