Separation and purification of triterpenes from Matteuccia struthiopteris with macroporous resin
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摘要: 目的:筛选出分离纯化荚果蕨总三萜的最佳大孔树脂型号及工艺条件。方法:以荚果蕨总三萜浓度为指标,通过静态和动态实验,筛选最佳大孔吸附树脂并初步确定总三萜纯化工艺。结果:AB-8型大孔树脂吸附解析效果最好,吸附条件:溶液浓度1.96mg/m L,p H为6,流速1.5m L/min,吸附体积5BV;洗脱条件:60%乙醇,流速2.0m L/min,洗脱体积4BV,其回收率为86.27%,纯度82.32%,精制倍数为2.88。结论:AB-8大孔树脂较适合分离纯化荚果蕨总三萜。该工艺简单可行,纯化效果好,可为工业生产中分离纯化荚果蕨总三萜提供理论指导和参考依据。Abstract: Objective:An optimal macroporous resin and process that to separate and purify triterpenes from Matteuccia struthiopteris was reasearched. Methods : The optimal resin and purificating technology were preliminary selected through the static and dynamic test, taking the concentration of triterpenes as the main index. Results:The best macroporous resin was AB-8, the adsorption conditions were described as follows:the p H and concentration of sample was 6 and 1. 96 mg / m L, the maximum injection volume was 5BV, the flow velocity was 1.5m L/min. The desorption process was determined as follows:60% ethanol was used as eluent at the flow rate of 2.0m L/min with 4BV, the recovery rate reached up to 86.27%, the purity was 82.32%, the purified multiples of triterpenes was 2.88. Conlusion:These data demonstrated the AB-8 macroporous resin in purifying triterpenes from Matteuccia struthiopteris was well. This method was easy to operate and displayed a good purification effect, the certain reference and theoretical guidance for separation and purification of triterpenes in industrial application could be provided.
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Keywords:
- Matteuccia struthiopteris /
- triterpenes /
- macroporous resin /
- separation /
- purification
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