Technological conditions optimization for GABA enrichment of germinated brown rice using Pulse light and salt stress method
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摘要: 为获得高含量γ-氨基丁酸（GABA）的发芽糙米,对盐胁迫下发芽糙米实施脉冲强光处理。在单因素实验基础上,采用Box-Behnken响应面法优化脉冲强光对盐胁迫下发芽糙米积累γ-氨基丁酸工艺条件。实验结果表明:谷氨酸钠浓度为2.01mg/m L、脉冲能量300J、脉冲距离11cm、脉冲频次1次/s、脉冲时间31.80s以及发芽时间27.84h时能达到最佳的积累条件,此条件下验证实验的发芽糙米中GABA含量可达到181.5mg/100g。Abstract: To obtain the high content of γ- aminobutyric acid of germinated brown rice, germinated brown rice was implemented pulsed light treatment on salt stress.On the basis of single factor experiment, Box- Behnken surface response was used for optimizing technological conditions of germinated brown rice on salt stress accumulated GABA by pulsed light.The experimental results indicated that Sodium glutamate concentration of 2.01 mg / m L, 300 J pulse energy, 11 cm pulse distance, once / s pulse frequency, 31.80 s pulse time and germination time of 27.84 h had the best enrichment. Under optimum conditions, the GABA content in germinated brown rice can be reached181.5mg /100 g.
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