Study on preparation and content determination of taraxacum polysaccharide selenide
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摘要: 根据有机硒化合物的合成原理,以蒲公英多糖与亚硒酸钠为原料制备蒲公英硒多糖。利用单因素和正交实验探讨最佳工艺条件;利用HPLC法测定蒲公英硒多糖中的硒含量,并通过红外光谱和差示扫描量热法对硒多糖进行了初步表征。结果表明,最佳工艺条件为蒲公英多糖与亚硒酸钠质量比为1∶1.2,硝酸体积分数为0.3%,反应温度70℃,反应时间8h。蒲公英硒多糖的平均硒含量为3.79mg/g,平均收率为33.58%。红外光谱显示:蒲公英硒多糖中含有Se=O键和Se-C键,实现了蒲公英多糖的硒化,可为进一步的开发和利用提供良好的条件。Abstract: The selenide taraxacum polysaccharide was prepared by using taraxacum polysaccharide and sodium selenite as the raw material, according to the synthesis principe of organic selenium compound. The optimal conditions were established by single factor and orthogonal design, the content of polysaccharide selenide was determined by HPLC, the structure of polysaccharide selenide were characterized by IR and DSC. The results showed that the mass ratio of taraxacum polysaccharide to sodium selenite was 1 ∶1.2, the volume fraction of HNO3 was 0.3%, the reaction temperature was 70℃ and the reaction time was 8 hours.Under the conditions, the average content of selenium in taraxacum polysaccharide was 3.79mg/g and average yield was 33.58%. Infrared Spectrum showed that the selenide taraxacum polysaccharide contained Se=O and Se-C, the selenization of taraxacum polysaccharide was realized, which provide a good condition for further development and utilization.
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Keywords:
- taraxacum polysaccharide /
- sodium selenite /
- preparation /
- characterization
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