一株木质纤维素酶高产菌株的选育及产酶条件研究
详细信息The development of high cellulase production mutant from Trichoderma Viride and study on the condition of enzyme production
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摘要: 主要通过紫外(UV)和甲基磺酸乙酯(EMS)的交替诱变的方法提高绿色木霉的产酶能力,并成功筛选到了一株产酶活性较高的菌株,其在平板筛选培养基中的透明圈直径与菌落直径之比达到2.1,传代稳定后在产酶培养基中纤维素酶活(CMCase)和滤纸酶活(FDAase)分别达到21.05U/mL和2.4U/mL。之后经培养基的优化进一步提高其酶活,最终诱变菌株在最佳产酶培养基中CMCase达到22.5U/mL,FDAase达到2.52U/mL。Abstract: The cellulase production strain T. viride 8140 was treated with UV and EMS. The mutant strain T.viride8140UEUE4-80 was showed to have a higher cellulase activity than others and the Hc of the mutant reached 2.1. After several passages its CMCase and FDAase were stabilized at 21.05U/mL and 2.4U/mL. The conditions of fermentation were studied and the optimal culture medium was decided. Under the optimal culture medium, the CMCase and FPAase of the mutant separately reached 22.5, 2.52U/mL.
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Keywords:
- T.viride /
- mutagenesis /
- cellulose /
- liquid fermentation
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[1] 邱向峰.纤维素酶高产菌株的选育及玉米秸秆糖化发酵乳酸的研究[D].青岛:中国海洋大学, 2006. [2] Hendriks A, Zeeman G.Pretreatments to enhance the digestibility of lignocellulosic biomass[J].Bioresource Technology, 2009, 100 (1) :10-18.
[3] LüJ L, Zhou P J.Optimal conditions for maximizing production of reducing sugars from microwave-assisted FeCl3pretreated rice straw degraded by Trichoderma viride and Bacillus pumilus[J].African Journal of Microbiology Research, 2011, 31 (5) :5757-5764.
[4] 肖舸.绿色木霉的筛选与产纤维素酶发酵条件优化及部分酶学性质研究[D].成都:四川大学, 2004. [5] 管斌.复合纤维素酶对杨木SGW浆纤维改性的应用及酶改性机理的研究[D].天津:天津轻工业学院, 1998. [6] Lan T Q, Wei D, Yang S T, et al.Enhanced cellulase production by<i>Trichoderma viride</i>in a rotating fibrous bed bioreactor[J].Bioresource Technology, 2013, 133:175-182..
[7] 李雪峰, 侯红萍.选育高产纤维素酶菌种的研究进展[J].酿酒科技, 2010 (5) :92-94. [8] Kovács K, Megyeri L, Szakacs G, et al.<i>Trichoderma atroviride</i>mutants with enhanced production of cellulase andβ-glucosidase on pretreated willow[J].Enzyme and Microbial Technology, 2008, 43 (1) :48-55.
[9] 高榕, 邓迎达.高生产效率纤维素酶菌株初筛方法的研究[J].纤维素科学与技术, 2004, 12 (3) :20-24. [10] Mandels M, Weber J.The production of cellulases[J].Adv Chem Ser, 1969, 95:391-414.
[11] 钱存柔, 黄义秀.微生物学实验教程[M].北京:北京大学出版社, 2008:36-57. [12] 沈萍, 范秀荣, 李光武.微生物学实验[M].第三版.北京:高等教育出版社, 2000:25-79. [13] Eveleigh D E, Mandels M, Andreotti R, et al.Measurement of saccharifying cellulase[J].Biotechnology for Biofuels, 2009, 2 (1) :21.
[14] 白洪志, 杨谦, 宋金柱.纤维素降解菌绿色木霉C-08产酶条件研究[J].哈尔滨工业大学学报, 2008, 40 (7) :1111-1115. [15] 邵喜霞, 韩大勇, 张力.绿色木霉合成纤维素酶部分培养条件的优化[J].安徽农业科学, 2009, 37 (23) :10893-10894. [16] Jiang X, Geng A, He N, et al.New isolate of<i>Trichoderma viride</i>strain for enhanced cellulolytic enzyme complex production[J].Journal of Bioscience and Bioengineering, 2011, 111 (2) :121-127.
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