Study on the composition and antioxidant activity of free and bound polyphenols in pomegranate peel
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摘要: 目的:研究石榴皮游离与结合态多酚含量、组成和抗氧化性的异同,为其提取和利用提供依据。方法:将3个石榴品种的成熟果实分别制成4类皮粉,采用超声提取法和碱液溶出法制取各皮粉的游离态和结合态多酚,采用3种方法测定其酚类组成和含量,采用4种方法评价其抗氧化能力。结果:4类皮粉的游离态多酚、类黄酮和单宁含量分别为107.55~157.60mg GAE/g DW、24.24~44.54mgQUE/gDW和143.03~231.44mgTAE/gDW,结合态多酚、类黄酮和单宁含量分别为7.35~30.89mgGAE/g DW、2.25~11.56mgQUE/gDW和9.01~18.86mgTAE/g DW。全皮、外层皮、内层皮和皮渣粉的多酚含量差异显著(p<0.05),其中皮渣游离态多酚含量最低而结合态多酚含量最高,各品种的同类皮粉的多酚含量有显著差别(p<0.05)。同一皮粉的游离态和结合态多酚的抗氧化能力彼此相近,其大小与多酚和单宁含量均显著相关(p<0.01)。结论:提取石榴皮多酚的材料可根据原料的实际状况而选全皮、内层皮或皮渣,提取游离酚可获取材料中大部分多酚。结合态多酚的抗氧化能力与游离态多酚相似但含量低。Abstract: Objective:To ascertain the contents, compositions and antioxidant activities of the free and the bound polyphenols of pomegranate peel for providing theoretical basis of extraction and utilization. Methods:Four kinds of peel power were prepared from pomegranate fruits of 3 cultivars respectively. Their free and bound polyphenols were extracted with ultrasonic-assisted extraction method, and caustic stripping method respectively. The polyphenol contents and compositions of the extracts were determined by 3 methods. The antioxidant activities of them were assayed by 4 methods. Results:The total polyphenol, total flavonoid, and total tannin contents of the free polyphenol extracts were in the ranges of 107.55~157.60 mg GAE/gDW, 24.24~44.54mgQUE/gDW and143.03~231.44mgTAE/gDW respectively. The total polyphenol, total flavonoid, and total tannin contents of the bound polyphenol extracts were in the ranges of 7.35~30.89mgGAE/gDW, 2.25~11.56mgQUE/gDW and 9.01~18.86 mg TAE/g DW respectively. The polyphenol compositions of whole peel, outer peel, inner peel, and juicing marc of whole peel powers were significantly different with each other (p<0.05) , and the power of juicing marc of whole peel had the lowest free polyphenols content and the highest bound polyphenols content among them.Meanwhile, the compositions of the extracts from same kind of peel power from the 3 cultivars were also significant different, which reflected the impact of the cultivar was also significant. In addition, the free polyphenol extract from any peel power had similar antioxidant capacity with the bound polyphenol extract from the same peel power. And the antioxidant capacity were in consistent significantly with both the total polyphenol contents and the total tannin contents of the extracts (p <0.01) . Conclusion :The raw material using for extracting pomegranate fruit peel polyphenols could be chosen from whole peel, the inner peel or the marc of peel according to their actual reserves and hygiene situation. Extraction of the free polyphenols could get most part of the polyphenols in the material. The antioxidant potential of the bound polyphenols of pomegranate peel was similar with the free ones at same dose, but the bound polyphenols content of peel was very lower generally.
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Keywords:
- pomegranate peel /
- free polyphenol /
- bound polyphenol /
- antioxidant capacity
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