Study on the effect of the protein from Kelp endophytes DNN6on preservation of Pseudosciaena polyactis
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摘要: 为了探究海带内生菌DNN6蛋白对水产品保鲜的效果,本文利用250μg/mL和500μg/mL的该蛋白对小黄鱼冷藏保鲜效果进行研究,以感官评分、细菌总数（TVC）、pH、挥发性盐基氮（TVB-N）和硫代巴比妥酸（TBA）等为鲜度指标来测定小黄鱼在冷藏过程中各种品质的变化。结果表明,在4℃冷藏条件下,当海带内生菌DNN6蛋白浓度为500μg/mL时,具有良好的抑菌效果,明显抑制细菌的生长繁殖,延缓挥发性盐基氮和TBA值的上升,延缓了小黄鱼的腐败。因此,海带内生菌DNN6蛋白在水产品保鲜领域具有一定应用前景。Abstract: To research the preservation effects of protein from Kelp endophytes DNN6 on aquatic products, the Pseudosciaena polyactis was treated with 250μg /mL and 500μg /mL of protein DNN6 respectively. The sensory score, total bacteria count, total volatile base nitrogen ( TVB-N) , pH value and the 2-thiobarbituric acid ( TBA) were used to evaluate the fish quality during cold storage. Results showed that 500μg /mL protein could inhibit the increase of total bacteria count, TVB- N and TBA. The protein DNN6 can delay the corruption of Pseudosciaena polyactis, and have a good prospect in preservation of aquatic products.
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Keywords:
- Kelp of endophyte /
- protein DNN6 /
- Pseudosciaena polyactis /
- antimicrobial activity /
- preservation
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[1] 郭旭鹏, 金显仕, 戴芳群.渤海小黄鱼生长特征的变化[J].中国水产科学, 2006, 13 (2) :243-249. [2] FAO.FIGIS-Web Site.Fisheries global information system (FIGIS) [R].FAO Fisheries and Aquaculture Department, Rome, 2010. (2010-07-13) [2010-08-11].
[3] 马妍, 谢晶, 周然.海水鱼类保鲜技术的研究进展[J].山西农业科学, 2011, 39 (6) :624-628. [4] 刘淑集, 吴成业, 刘智禹.水产品生物保鲜技术的应用及展望[J].天津农业科学, 2012, 18 (5) :46-50. [5] 蓝蔚青, 谢晶, 赵海鹏, 等.茶多酚对冷藏带鱼保鲜效果的比较研究[J].湖北农业科学, 2010, 49 (1) :159-162. [6] Jeon Y J, Kamil J Y, Shahidi F.Chitosan as an edible invisible film for quality preservation of herring and atlantic cod[J].Journal Agricultural Food Chemistry, 2002, 50 (18) :5167-5178.
[7] 张瑛.海带共附微生物的分离及活性研究[D].大连:大连海洋大学, 2011. [8] GB 4789.2-2010, 菌落总数测定食品安全国家标准[S]. [9] Arashisar S, Hisar O, Kayb M, et al.Effects of modified atmosphere and vacuum packaging on microbiological and chemical properties of rainbow trout (Oncorynchus mykiss) fillets[J].Food Microbiology, 2004 (97) :209-214.
[10] 马妍, 谢晶, 周然, 等.冷藏温度对河豚鱼微生物及品质特性的影响[J].食品科学, 2012, 33 (6) :288-292. [11] SIU G M, DRAPER H.A survey of the malonaldehyde content of retail meats and fish[J].Journal of Food Science, 1978, 43 (4) :1147-1149.
[12] 张旭光, 李婷婷, 朱军莉, 等.茶多酚处理对冷藏养殖大黄鱼品质的影响[J].茶叶科学, 2011, 31 (2) :105-111. [13] 仲济健, 卢立新.真空包装淡腌白鱼的辐照保藏研究[J].食品工业科技, 2012, 33 (13) :336-338. [14] Esteves E, Anibal J.A sensory analysis-based method of determining fish quality[J].Food Convention, 2006 (11) :1-7.
[15] 励建荣, 李婷婷, 李学鹏.水产品鲜度品质评价方法研究进展[J].北京工商大学学报:自然科学版, 2010, 128 (16) :1-8. [16] 汪之和.水产品加工与利用[M].北京:化学工业出版社, 2002. [17] 兀征, 王登临, 董瑞鹏, 等.LAL实验快速检测肉品细菌污染程度及鲜度的研究[J].中国预防兽医学报, 2002, 24 (4) :308. [18] Jo C, Ahn D U.Volatiles and oxidatives changes in irradiated pork sausage with different fatty acid composition and tocopherol content[J].Journal of Food Science, 2000, 65 (2) :270-275.
[19] Ruiz-Capillas C, Moral A.Changes in free amino acids during chilled storage of hake (Merluccius merluccius, L.) in controlled atmospheres and their use as a quality control index[J].European Food Research and Technology, 2001, 212:302-307.
[20] 高华, 刘坤, 朱明.新型鱼类保鲜剂保鲜效果研究[J].青岛大学学报, 2000, 15 (4) :11-14. [21] 张旭光.茶多酚结合壳聚糖处理对冷藏养殖大黄鱼保鲜效果研究[D].杭州:浙江工商大学, 2011.
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