Abstract: Six strains of Bacillus coagulan were studied for their ability of thermotolerant and production of lactic acid with corn meal.Four strains of B.coagulan ( HL1, HL3, HL4, HL5) had been screened from plate culture. Then they were cultured at 50, 53 and 55℃ respectively, the results showed that HL5 was more thermotolerant. In 5L batch fermentation with corn saccharification liquid as carbon source at 50℃, HL5 had the highest L- lactic acid production, productivity and yield, which were 115g /L, 3.59g /L /h, and 100.1%, respectively.At last, comparisions of L-lactic acid production with corn meal, cassava flour and cellulose as carbon sources were carried out, and the productivity were 4.19g /L /h, 3.75g /L /h, 5.00g /L /h respectively, indicating these carbon sources could be utilized by HL5.Therefore HL5 is qualified as a potential industry lactic acid producer.